Expression of heme oxygenase in pulmonary arteries of rats with hypoxic pulmonary hypertension

王关嵩; 钱频; 李琦; 李运成; 程序; 周长喜; 张志远; 钱桂生

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Expression of heme oxygenase in pulmonary arteries of rats with hypoxic pulmonary hypertension

更新时间：2026-01-09

- Expression of heme oxygenase in pulmonary arteries of rats with hypoxic pulmonary hypertension
- Journal of Army Medical University Vol. 31, Issue 15, Pages: 1428-1431(2009)
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  CLC： R345;R363.25;R543.2
- Published：2009
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王关嵩,钱频,李琦,李运成,程序,周长喜,张志远,钱桂生.低氧肺动脉高压时血红素氧合酶基因表达状况的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(15):1428-1431.

　WANG Guan-song,QIAN Pin,LI Qi,LI Yun-cheng,CHENG Xu,ZHOU Chang-xi,ZHANG Zhi-yuan,QIAN Gui-sheng.Expression of heme oxygenase in pulmonary arteries of rats with hypoxic pulmonary hypertension[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(15):1428-1431.
王关嵩,钱频,李琦,李运成,程序,周长喜,张志远,钱桂生.低氧肺动脉高压时血红素氧合酶基因表达状况的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(15):1428-1431. DOI：

　WANG Guan-song,QIAN Pin,LI Qi,LI Yun-cheng,CHENG Xu,ZHOU Chang-xi,ZHANG Zhi-yuan,QIAN Gui-sheng.Expression of heme oxygenase in pulmonary arteries of rats with hypoxic pulmonary hypertension[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(15):1428-1431. DOI：

摘要

目的探讨血红素氧合酶（heme oxygenase

HO）基因在低氧肺动脉高压大鼠肺动脉组织中的表达及一氧化碳（carbon monoxide，CO）对其的影响。方法 60只 Wistar大鼠随机分为5组：常氧组；低氧肺动脉高压组；血晶素组；锡原卟啉组；低浓度CO组（n=12）。采用紫外分光光度计、逆转录-聚合酶链反应、免疫组织化学染色和原位杂交进行检测。结果 ①HO-1活性：低氧组、血晶素组、低浓度CO组肺动脉组织HO-1活性以胆红素生成量计算，均升高，其中血晶素组最高，与正常对照组比较，差异有显著性(P＜0.01)。②逆转录-聚合酶链反应：各组大鼠肺动脉HO-2 mRNA表达没有明显变化，均保持稳定。缺氧组、血晶素组、锡原卟啉组和低浓CO组HO-1 mRNA均明显高于正常对照组，以低浓度CO组升高最明显(P＜0.01)。血晶素组和低浓度CO组均显著高于缺氧组(P＜0.01)，而锡原卟啉组低于缺氧组。③免疫组织化学染色：低氧组肺动脉内膜、中膜的HO-1表达均明显升高，血晶素组和低浓度CO组更高，锡原卟啉组和常氧组均有很微弱表达。④原位杂交：低氧组肺动脉3层均加深2～3级

低浓度CO组肺动脉外、中、内膜细胞质均明显加深4级。处理前后HO-2的染色变化不大。结论低氧肺动脉高压时，肺动脉组织HO-1基因的表达升高，可能在肺动脉高压的发生和发展中起一定作用。

Abstract

Objective To investigate the expression of heme oxygenase (HO) in pulmonary arteries of rats with hypoxic pulmonary hypertension (HPH). Methods A total of 60 rats were employed and equally divided into 5 groups (n=12 in each group)

i. e. control (group Ⅰ)

HPH (group Ⅱ)

hemin (group Ⅲ)

tin protoporphyrin (SnPP) (group Ⅳ)

and low concentration CO group (group Ⅴ). Of them

the rats from middle 3 groups were treated with hypoxia under normal pressure for 7 h

in every day except Sunday

consecutively for 3 weeks. Hemin and tin protoporphyrin (SnPP) were given half an hour before hypoxia while low concentration CO at 2 h after hypoxia. HO-1 activity of pulmonary arteries tissues was tested with ultraviolet spectrophotometry. The expression of HO was detected with reverse transcriptionpolymerase chain reaction (RT-PCR)

immunohistochemical staining and in situ hybridization. Results ① The bilirubin production increased significantly in group [(396.28±69.62) pmol·mg

-1

·h

-1

]

group [(668.56±189.29) pmol·mg

-1

·h

-1

]

group [(267.38±88.59) pmol·mg

-1

·h

-1



]

and group [(589.32±132.35) pmol·mg

-1.

-1

]

as compared with those in group [(125.62±31.52) pmol·mg

-1

·h

-1

]

. ② The levels of HO-1 mRNA expression of pulmonary arteries in group Ⅱ

group Ⅲ

group Ⅳ and group Ⅴ were significantly higher than those in group Ⅰ. The most significant HO-1 mRNA expression was found in group Ⅴ (P0.01). The levels of HO-1 mRNA expression of pulmonary arteries in group Ⅲ and group Ⅴ were higher than those in group Ⅱ

but it was lower in group Ⅳ than that in group Ⅱ. ③ The strong positive granules of HO-1 were observed in pulmonary arteries intima and media in group Ⅱ

group Ⅲ and group Ⅳ

while group Ⅲ and group Ⅳ involved more positive granules of HO-1 as compared with those in group Ⅱ. ④ The staining was deepened in three layers of the pulmonary arteries in group Ⅱ

while the staining was deepened more in group Ⅲ and group Ⅳ. No significant change was found in staining of HO-2 in different groups. Conclusion CO can up-regulate the expression of HO-1

and this may play important roles in the genesis and development of pulmonary hypertension.

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