Objective    To investigate CD14 binding site with lipopolysaccharide binding protein (LBP) by phage peptide library display

DNAStar software analyses

affinity test and LBP competitive inhibition experiments.     Methods    With CD14 as a target molecule

phage peptide library display

phage ELISA

LBP competitive inhibition experiments and DNA screening for testing sequences were jointly adopted to deduct the display peptide sequences of the binding site of LBP/CD14. The display peptide sequences were matched with primary structure of LBP by DNAStar analysis software

to initially locate CD14 binding site with LBP and synthesis its mimic peptide. The affinity activity and competitive inhibition activity of mimic peptide were detected by ELISA.     Results    DNAStar software analyses indicated that the sequences of 8 peptides obtained by phage peptide library were similar to 252 to 263 amino acids of LBP

and satisfactory hydrophilicity

accessibility

flexibility and antigenicity were seen in this amino acid fragment of LBP. The binding site of LBP/CD14 might locate in this fragment. In vitro activity test indicated that mimic peptide (FHRNHRSPVTLL) had satisfactory affinity activity to CD14 and competitive inhibition activity to LBP.     Conclusion    The mimic peptide (FHRNHRSPVTLL) of the 252 to 263 amino acid fragment of LBP has satisfactory affinity activity to CD14 and competitive inhibition activity to LBP. It displays the bioactivity of the binding site of LBP/CD14 and indicates CD14 binding site with LBP is locate in this region preliminarily.