Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1<sub>265-2493</sub> and three copies of C3d

梁培禾; 张克勤; 聂志林; 许桂莲; 李彦锋; 叶锦; 靳风烁

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Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1_265-2493 and three copies of C3d

更新时间：2026-01-09

- Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1_265-2493 and three copies of C3d
- Journal of Army Medical University Vol. 31, Issue 13, Pages: 1239-1342(2009)
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  CLC： R392-33;R392.2;R392.7
- Published：2009
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梁培禾,张克勤,聂志林,许桂莲,李彦锋,叶锦,靳风烁.Flk-1265-2493与C3d3基因融合质粒诱导小鼠免疫应答的初步研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(13):1239-1342.

　LIANG Pei-he,ZHANG Ke-qin,NIE Zhi-lin,XU Gui-lian,LI Yan-feng,YE Jin,JIN Feng-shuo.Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1265-2493 and three copies of C3d[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1239-1342.
梁培禾,张克勤,聂志林,许桂莲,李彦锋,叶锦,靳风烁.Flk-1265-2493与C3d3基因融合质粒诱导小鼠免疫应答的初步研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(13):1239-1342. DOI：

　LIANG Pei-he,ZHANG Ke-qin,NIE Zhi-lin,XU Gui-lian,LI Yan-feng,YE Jin,JIN Feng-shuo.Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1265-2493 and three copies of C3d[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1239-1342. DOI：

摘要

目的对pSG.SS.Flk-1

265-2493

.C3d3.YL及pSG.SS.Flk-1

265-2493

.YL真核表达质粒的免疫效应进行初步研究。方法构建pSG.SS.Flk-1

265-2493

.C3d3.YL及pSG.SS.Flk-1

265-2493

.YL真核表达质粒后，48只BALB/c小鼠随机均分为疫苗组、疫苗对照组、质粒对照组、空白对照组（12只/组），分别肌肉注射接种此2种质粒、pSG.SS.YL质粒和PBS。免疫后动物：①每组6只取脾脏分离细胞，MTT法测定经Flk-1蛋白刺激后的增殖效应，乳酸脱氢酶释放法测定对培养小鼠肺微血管内皮细胞的杀伤作用；②其余6只动物于不同时间点断尾取血分离血清，ELISA法测定抗Flk-1抗体水平的动态变化。结果经Flk-1蛋白刺激，疫苗组小鼠脾淋巴细胞的增殖效应明显较强，刺激指数显著高于其他3组（P均0.01），对靶细胞（MVECs）的杀伤活性在不同效/靶比时均显著高于其他3组（P均0.01）。疫苗组动物血清抗Flk-1抗体比对照疫苗组抗体阳性反应出现早2周，二者峰值效价均出现在5周（血清稀释度：1∶3840 vs 1∶60），前者12周时仍保持较高水平（1∶960），后者10周时抗体阳性反应已消失。结论 C3d3的连接有效增强了Flk-1

265-2493

的免疫原性。

Abstract

Objective To evaluate the immunological effect of eukaryotic expression plasmids pSG.SS.Flk-1

265-2493

.C3d3.YL and pSG.SS.Flk-1

265-2493

.YL. Methods After construction of eukaryotic expression plasmids pSG.SS.Flk-1

265-2493

.C3d3.YL and pSG.SS.Flk-1

265-2493

.YL

BALB/c female mice (n=48) were separated into four groups (n=12 per group) and were in

oculated by intramuscular injection of the two plasmids

pSG.SS.YL plasmid (plasmid control) and PBS (blank control)

respectively. After inoculation

proliferation of spleen lymphocytes stimulated with Flk-1 protein was studied using MTT method and the specific cytotoxicity T lymphocyte (CTL) effect on target cells (microvascular endothelial cells

MVECs) was assayed with lactate dehydrogenase-release method. Then anti-Flk-1 antibody titers in the immune sera were assayed with indirect enzyme-linked immunosordent assay (ELISA). Results After stimulation with Flk-l antigen

the proliferation and specific CTL activities of spleen lymphocytes in mice immunized with pSG.SS.Flk-1

265-2493

.C3d3.YL were obviously stronger than those in other three groups. Although both plasmids could elicit Flk-1 specific antibody

in pSG.SS.Flk-1

265-2493

.C3d3.YL immunized mice

the antibody presented 2 weeks earlier

and the titer was significantly higher than that in mice immunized with pSG.SS.Flk-1

265-2493

.YL. Conclusion The results show that the C3d3 fusion strategy can enhance the immunogenicity of Flk-1 significantly

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Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1265-2493 and three copies of C3d

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摘要

Abstract

关键词

Keywords

references

Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1_265-2493 and three copies of C3d