Objective    To establish an obesity animal model in mice and evaluate the model.     Methods    A total of 40 clean 3-week-old weaning female C57BL/6J mice were randomly and equally divided into 4 groups. Group A were fed with normal diet food and sensitized with normal saline (NS)

group B were fed with normal diet and challenged with ovalbumin (OVA). Groups C and D were fed with high fat diet and only the later received OVA challenging. Body weight

liver weight

index of liver/body weight

Lee’s index

lung morphology

cell number of bronchoalveolar lavage fluid

concentration of IL-4 and IFN-γ were measured or calculated.     Results    (1) The body weight

liver weight

index of liver/body weight

Lee’s index of group C and D were significantly higher than those of group A and B. (2) Local inflammation was significant in groups B and D

with the later more serious. (3) Total cell count and ratio of eosinophil of bronchoalveolar lavage fluid were highest in group D followed by group B and group A. (4) Groups B and D had a higher concentration of IL-4 and lower level of IFN-γ in comparison with group A and C

and the ratio of IL-4/IFN-γ was higher in group D than group B. (5) Two mice respectively from groups C and D had not meet the obesity criterion before challenge

and the other 2 mice from group B and D respectively died during challenge

so they were excluded in this study.     Conclusion    Mouse obesity asthma animal model can be established in C57BL/6J mice by feeding with self-made high fat food and sensitized and provocated by OVA.