Objective    To observe the changes of F-actin of strial capillary endothelial cells from guinea pig cochlea after TNF-α  treatment so as to further study the mechanism of permeability of those cells.     Methods    Strial capillary endothelial cells were dissociated from guinea pig cochlea and cultured respectively with 0.05

0.1

0.2 ng/ml TNF-α for 90 min

then detected by immunofluorescence laser confocal microscopy for F-actin concentrations. The blank control was set simultaneously.     Results    TNF-α decreased the content of F-actin in strial capillary endothelial cells (P0.01)

and with the increasing TNF-α concentration

the F-actin concentration declined.     Conclusion    TNF-α may change the density and content of F-actin in strial capillary endothelial cells of guinea pig cochlea.