Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells

张颖; 杨柳; 段小军; 王富友

您当前的位置：

首页 >

文章列表页 >

Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells

更新时间：2026-01-09

- Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells
- Journal of Army Medical University Vol. 30, Issue 21, Pages: 2041-2043(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R331.1;R331.2
- Published：2008
- 稿件说明：
移动端阅览
张颖,杨柳,段小军,王富友.不同浓度羊自体血清对骨髓间充质干细胞增殖能力影响的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(21):2041-2043.

　ZHANG Ying,YANG Liu,DUAN Xiao-jun,WANG Fu-you.Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(21):2041-2043.
张颖,杨柳,段小军,王富友.不同浓度羊自体血清对骨髓间充质干细胞增殖能力影响的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(21):2041-2043. DOI：

　ZHANG Ying,YANG Liu,DUAN Xiao-jun,WANG Fu-you.Effect of goat autologous serum at different concentrations on proliferation of bone marrow-derived mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(21):2041-2043. DOI：

摘要

目的观察不同浓度羊自体血清对骨髓间充质干细胞（bone marrow-derived mesenchymal stem cells

bMSCs）增殖活力的影响，并筛选出最佳血清培养浓度。方法以12月龄青山羊为实验动物，首先制备自体血清，分离获取bMSCs；然后选DMEM/F12为基础培养基，采用不同体积分数（分别为5%、10%、15%）自体血清及10%体积分数胎牛血清培养基分别对bMSCs进行培养；通过显微镜下细胞形态学观察、活细胞计数及MTT等方法对细胞进行检测，并对检测数据做统计学分析。结果不同浓度自体血清培养培养条件下bMSCs形态正常。与10%胎牛血清组进行比较，5%自体血清组bMSCs增殖较慢（P0.01），10%、15%自体血清组增殖较好，但组间无统计学差异（P0.05）。结论羊自体血清培养bMSCs是可行的，10%浓度自体血清是最佳培养浓度。

Abstract

Objective To investigate the effect of goat autologous serum at different concentrations on the proliferation of bone marrow-derived mesenchymal stem cells (bMSCs) for optimal serum dose. Methods Autologous serum was prepared from 6 goats at 12 months old

and their bMSCs were isolated from bone marrow. Then the obtain bMSCs were cultured in DMEM/F12 medium with supplement of 5%

10%

or 15% of corresponding autologous serum

and 10% fetal bovine serum served as control. The morphology of bMSCs

cells counting and MTT assay were observed or carried out. Results Morphology of the bMSCs in the different groups were similar. The proliferation of 5% autologous serum group was slower compared with that of 10% fetal bovine serum group (P0.05). Both the 10% and 15% autologous serum groups had the similar proliferation as that of 10% fetal bovine serum group. Conclusion Cultivation of bMSCs with autologous serum is feasible. The optimized concentration of autologous serum is 10%.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Specific expression of reporter gene FTH1 driven by progression elevated gene-3 promoter in malignant transformation of rat mesenchymal stem cells and its detection by in vitro magnetic resonance imaging

HGF promotes osteogenic differentiation in mesenchymal stem cells induced by bone morphogenetic protein 9

Role of Notch1 signaling in the process of BMP9-induced osteogenetic differentiation of immortalized mouse embryonic fibroblasts

Effects of different-diameter TiO₂ nanotubes on proliferation and osteogenic differentiation of rat mesenchymal stem cells

PDTC promotes wound healing in STZ-reduced diabetic mice by mesenchymal stem cells

Related Author

仇嘉文

李晓朦

秦勇

蔡金华

孙君

毕杨

罗聪

惠慧

Related Institution

No data

⁰