Objective    To observe the influence of the bone marrow microenvironment of normal or acute lymphoblastic leukemia on biological characteristics of Jurkat cells.     Methods    We collected the Jurkat cells which had been co-cultured with bone marrow stromal cells from 6 cases of acute lymphoblastic leukemia

2 cases of iron deficiency anemia

or 2 cases of  idiopathic thrombocytopenic purpura  for 10 d

and then observe the changes of Jurkat cells’ proliferation

cell cycle

apoptosis

differentiation and daunorubicin (DNR) intake dose.     Results    Leukemia bone marrow stromal cells promoted Jurkat cells’ adhesion

chemotaxis and reduction of intake dose than normal bone marrow stromal cells; but were weaker in inhibition of proliferation and promotion of differentiation. Leukemia bone marrow stromal cells inhibited Jurkat cells’ apoptosis

and the normal bone marrow stromal cells promoted apoptosis.     Conclusion    The micro-environment of normal bone marrow stromal cells promote the apoptosis and differentiation

and inhibit the proliferation of Jurkat cells

in comparison with leukemia bone marrow stromal cells

and increase Jurkat cells’ sensitivity to the DNR.