Objective    To investigate the killing effects of Fcy∶∶Fur suicide gene therapy system on human ovarian cancer cells (SKOV3).     Methods    The EGFP(enhanced green fluorescent protein)-labeled eukaryotic expression plasmid encoding Fcy∶∶Fur suicide gene fragment was constructed. The vector was transferred into SKOV3 cells. The stable transfectants were screened and analyzed by RT-PCR and Western blot analysis for the target gene expression．The killing efficacy of 5-FC on the transfected cells was determined by MTT assays and flow cytometry.     Results    Correct construction of pEGFP-N1-Fcy∶∶Fur was identified by nucleotide sequencing. About 60％ transfected cells displayed green fluorescence after 24 hours’ transfection. RT-PCR and Western blotting indicated that the transfected cells expressed Fcy∶∶Fur gene. MTT and flow cytometry showed 5-FC killed transfected SKOV3 cells.     Conclusion    Fusion suicide gene therapy system Fcy∶∶Fur/5-FC has a significant efficacy to kill human ovarian cancer cell line SKOV3.