Influence of inhibited ubiquitin proteasome system to heat shock protein 27 and tumor necrosis factor-α in cardiac ischemia-reperfusion rat

戴翠莲; 罗开良; 陈章荣; 赵静

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Influence of inhibited ubiquitin proteasome system to heat shock protein 27 and tumor necrosis factor-α in cardiac ischemia-reperfusion rat

更新时间：2026-01-09

- Influence of inhibited ubiquitin proteasome system to heat shock protein 27 and tumor necrosis factor-α in cardiac ischemia-reperfusion rat
- Journal of Army Medical University Vol. 30, Issue 22, Pages: 2115-2119(2008)
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  CLC： R322.11;R362;R654.102
- Published：2008
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戴翠莲,罗开良,陈章荣,赵静.抑制UPS对缺血再灌注大鼠心肌Hsp27和TNF-α的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(22):2115-2119.

　DAI Cui-lian,LUO Kai-liang,CHEN Zhang-rong,ZHAO Jing.Influence of inhibited ubiquitin proteasome system to heat shock protein 27 and tumor necrosis factor-α in cardiac ischemia-reperfusion rat[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):2115-2119.
戴翠莲,罗开良,陈章荣,赵静.抑制UPS对缺血再灌注大鼠心肌Hsp27和TNF-α的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(22):2115-2119. DOI：

　DAI Cui-lian,LUO Kai-liang,CHEN Zhang-rong,ZHAO Jing.Influence of inhibited ubiquitin proteasome system to heat shock protein 27 and tumor necrosis factor-α in cardiac ischemia-reperfusion rat[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):2115-2119. DOI：

摘要

目的研究泛素-蛋白酶体系统（ubiquitin-proteasome system， UPS）对心肌缺血再灌注大鼠热休克蛋白27（heat shock protein，Hsp27）的影响及与肿瘤坏死因子-α(TNF-α）的关系，探讨抑制UPS后可能的心肌保护作用机制，为降低心肌缺血再灌注损伤提供新的干预途径。方法 64 只SD大鼠结扎左冠状动脉前降支 30 min制作心肌缺血再灌注模型。缺血再灌注+治疗（I/R+T）组于再灌注前 5 min静脉注射蛋白酶体抑制剂MG-132 0.75 mg/kg，缺血再灌注（I/R）组、缺血（I）组及假手术（Sham）组注射与之相同容积的生理盐水。观察各组心肌梗死范围、再灌注6 h后各组大鼠心肌组织Hsp27 及TNF-α的表达。结果与I/R组相比，I/R+T组大鼠心肌梗死范围明显减少（P0.05），Hsp27 表达显著增加［mRNA水平分别为（95.37±18.38）和（68.14±17.58），P0.01；蛋白质积分光密度值分别为（82.57±6.39）和（39.96±7.28），P0.001］。TNF-α表达降低［mRNA分别为（45.53±10.65）和（76.52±19.12），P0.01；蛋白质积分光密度值分别为（60.12 ± 9.32）和（42.33 ± 5.95），P0.01］。结论适当抑制UPS能够显著升高Hsp27的水平，抑制TNF-α表达

减少心肌梗死范围

具有心肌保护作用。

Abstract

Objective To study the influence on heat shock protein 27 (Hsp27) and tumor necrosis factor α (TNF-α) by inhibiting ubiquitin proteasome system in cardiac ischemia-reperfusion (I/R) rats. Methods Left anterior descending (LAD) coronary artery was ligated for 30 min and then released for 6 h in adult Sprague-Dawley rats. Five minutes before reperfusion

MG-132 (N-benzoyloxycarbonyl (Z)-Leu-Leuleucinal) at dose of 0.75 mg/kg was given by vein injection in I/R+T group

but I/R group

I group and sham group were given the same volume of sterile saline. The changes of myocardial infarct size

cardiac protein and mRNA levels of Hsp27 and TNF-α were measured. Results Compared with I/R group

the myocardial infarct size was significantly reduced (P0.05)

while the protein and mRNA levels of Hsp27 were significantly increased (P0.01) and those of TNF-α were significantly lessened (P0.01) in I/R+T group. Conclusion Myocardial reperfusion injury can be inhibited by appropriate inhibition of UPS

during which the level of Hsp27 was increased and the expression of TNF-α was suppressed.

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