Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells

罗颖; 温贤浩; 徐酉华

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Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells

更新时间：2026-01-09

- Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells
- Journal of Army Medical University Vol. 30, Issue 23, Pages: 2215-2218(2008)
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  CLC： R331.144;R73-362;R733.7
- Published：2008
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罗颖,温贤浩,徐酉华.K562细胞survivin基因表达对淋巴细胞增殖和功能的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(23):2215-2218.

　LUO Ying,WEN Xian-hao,XU You-hua.Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(23):2215-2218.
罗颖,温贤浩,徐酉华.K562细胞survivin基因表达对淋巴细胞增殖和功能的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(23):2215-2218. DOI：

　LUO Ying,WEN Xian-hao,XU You-hua.Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(23):2215-2218. DOI：

摘要

目的探讨K562细胞中survivin 基因的表达对淋巴细胞增殖和功能的影响。方法将融合重组质粒pEGFP

survivin 和靶向survivin基因的RNAi的重组质粒pTZU6

survivin分别转染K562细胞，得到K562/survivin

和 K562/survivin

2种细胞，用G418筛选K562/survivin

细胞，用半定量逆转录-聚合酶链反应(RT-PCR)方法及免疫细胞化学法检测K562/survivin

和K562/survivin

2种细胞中survivin mRNA及蛋白水平的表达，并与K562细胞作对照。将这3种细胞与健康人外周血单个核细胞（PBMC）混合培养（MLTC），检测混合培养体系中淋巴细胞增殖能力、NK细胞活性及培养上清中IFN-γ水平。结果 K562/survivin

组的淋巴细胞增殖指数明显低于其余两组。survivin基因的表达水平与NK细胞活性呈负相关。混合培养上清中分泌的IFN-γ，K562/survivin

组是K562组的1/4.5，是K562/survivin

组的1/8。结论高表达的survivin可以抑制淋巴细胞的增殖、NK细胞活性及IFN-γ的分泌。

Abstract

Objective To study the effect of survivin gene expression on the lymphocyte’s proliferation and function in cultured K562 cells. Methods The constructed recombinant vector pEGFP-C1-survivin and the plasmid pTZU6

1-survivin encoding short hairpin RNA of survivin were transfected into K562 cells respectively to generate K562/survivin

cells and K562/survivin

cells. K562/survivin

cells were selected by G418. The survivin mRNA and protein levels in the 3 kinds of cells (K562/survivin

K562 and K562/survivin

) were detected by semi-quantitative RT-PCR and immunohistochemistry methods. The peripheral blood mononuclear cells (PBMC) from healthy subjects were co-cultured with these 3 cells respectively in mixed lymphocyte-tumor cell culture (MLTC). Lymphocyte proliferation in the supernatant was evaluated by MTT assay. Nature killer activity was detected by flow cytometry and IFN-γ level was measured by ELISA assay. Results Proliferation index in K562/survivin

group was much lower than that in the other 2 groups (P＜0.05). Survivin expression level was of negative correlation with NK activity. IFN-γ content in the MLTC of K562/survivin

group was lower than that in K562 group (1/4.5) and K562/survivin

group (1/8). Conclusion Enhanced expression of survivin may inhibit the proliferation of lymphocyte

activity of nature killer and the secretion of IFN-γ.

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