Rosiglitazone reduces no-reflow area following reperfusion after AMI by inhibiting adhesion molecules in rats

何泉; 雷寒; 覃数; 马康华

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Rosiglitazone reduces no-reflow area following reperfusion after AMI by inhibiting adhesion molecules in rats

更新时间：2026-01-09

- Rosiglitazone reduces no-reflow area following reperfusion after AMI by inhibiting adhesion molecules in rats
- Journal of Army Medical University Vol. 31, Issue 2, Pages: 128-131(2009)
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  CLC： R392.32;R542.22;R977
- Published：2009
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何泉,雷寒,覃数,马康华.罗格列酮抑制粘附分子表达减轻大鼠急性心肌梗死再灌注后无再流[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):128-131.

　HE Quan,LEI Han,QIN Shu,MA Kang-hua.Rosiglitazone reduces no-reflow area following reperfusion after AMI by inhibiting adhesion molecules in rats[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):128-131.
何泉,雷寒,覃数,马康华.罗格列酮抑制粘附分子表达减轻大鼠急性心肌梗死再灌注后无再流[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):128-131. DOI：

　HE Quan,LEI Han,QIN Shu,MA Kang-hua.Rosiglitazone reduces no-reflow area following reperfusion after AMI by inhibiting adhesion molecules in rats[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):128-131. DOI：

摘要

目的观察罗格列酮对大鼠急性心肌梗死（acute myocardial infarction，AMI）再灌注后无再流的影响及其机制。方法 45只SD大鼠分为假手术组、生理盐水组、罗格列酮组（3 mg·kg

-1

·d

-1

共14 d），每组15只，后2组大鼠行冠状动脉结扎3 h，松解2 h建立AMI再灌注后无再流模型。以病理染色法检测无再流面积；化学比色法检测再灌注区心肌髓过氧化物酶（myeloperoxidase， MPO）活性；以免疫组化及RT-PCR方法检测再灌注区细胞间粘附分子-1（intercellular adhesion molecule-1，ICAM-1）和P-选择素蛋白及mRNA的表达。结果 ①罗格列酮组心肌无再流范围较生理盐水组显著减小（P0.01）；②生理盐水组心肌MPO酶活性、ICAM-1及P-选择素蛋白及mRNA表达水平较假手术组均显著升高（P0.01）

罗格列酮组上述指标较生理盐水组显著降低（P0.05）。结论罗格列酮能有效减小大鼠急性心肌梗死再灌注后无再流范围，其机制可能与抑制再灌注后内皮ICAM-1及P-选择素的表达，进而减少中性粒细胞的粘附及聚积有关。

Abstract

Objective To investigate the mechanism by which rosiglitazone affects no-reflow following reperfusion after acute myocardial infarction (AMI). Methods Forty-five SD rats were randomly divided into sham-operated group

normal saline treated group and rosiglitazone treated group (at dose of 3 mg·kg

-1

·d

-1

for 14 d)

15 rats in each group. To establish no-reflow model

the rats of the latter two groups received coronary artery ligation for 3 h and reopening for 2 h. Then pathological sta

ining for no-reflow area

chemical assay for MPO activity

immunohistochemistry and RT-PCR for protein and mRNA expressions of ICAM-1 and P-selectin in myocardium were performed. Results No-reflow area in rosiglitazone treated group was significantly reduced

compared with that in normal saline treated group (P0.01). The MPO activity

protein and mRNA expressions of ICAM-1 and P-selectin in normal saline treated group were significantly increased (P0.01)

compared with that in sham-operated group

whereas those were significantly inhibited (P0.05) in rosiglitazone treated group. Conclusion Rosiglitazone can effectively reduce no-reflow area following reperfusion after AMI in rats

probably through downregulating ICAM-1 and P-selectin expressions in the myocardium after reperfusion and thereby further inhibiting adhesion and accumulation of neutrophil.

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