Construction of an eukaryotic vector expressing maspin gene and its effect on proliferation of gastric cancer cell line SGC7901

郑爱华; 易永芬; 周文文

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Construction of an eukaryotic vector expressing maspin gene and its effect on proliferation of gastric cancer cell line SGC7901

更新时间：2026-01-09

- Construction of an eukaryotic vector expressing maspin gene and its effect on proliferation of gastric cancer cell line SGC7901
- Journal of Army Medical University Vol. 31, Issue 4, Pages: 326-329(2009)
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  CLC： R394-33;R730.23;R735.2
- Published：2009
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郑爱华,易永芬,周文文.MASPIN真核表达载体的构建及其对胃癌细胞株SGC7901增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(04):326-329.

　ZHENG Ai-hua,YI Yong-fen,ZHOU Wen-wen.Construction of an eukaryotic vector expressing maspin gene and its effect on proliferation of gastric cancer cell line SGC7901[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(04):326-329.
郑爱华,易永芬,周文文.MASPIN真核表达载体的构建及其对胃癌细胞株SGC7901增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(04):326-329. DOI：

　ZHENG Ai-hua,YI Yong-fen,ZHOU Wen-wen.Construction of an eukaryotic vector expressing maspin gene and its effect on proliferation of gastric cancer cell line SGC7901[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(04):326-329. DOI：

摘要

目的构建MASPIN真核表达载体，分析MASPIN过表达对胃腺癌细胞株SGC7901的影响。方法 PCR扩增MASPIN基因全长，用载体PCR2.1构建重组质粒，转染至胃癌细胞株SGC7901。RT-PCR、Western blot检测MASPIN基因表达变化，MTT法检测细胞增殖情况，流式细胞仪（FCM）分析细胞周期，观察过表达MASPIN对SGC7901细胞株的影响。结果成功构建MASPIN/ PCR2.1表达载体并转染至胃癌细胞株SGC7901，转染上调了SGC7901细胞株MASPIN在mRNA、蛋白水平的表达。转染MASPIN的SGC7901细胞增殖率随时间而减慢，第24、48、72小时的细胞增殖率分别为93.07％、81.97％、66.5％，明显低于转染空载体组（95.98％、95.79％、95.59％，P0.05）。与转染空载体组相比，过表达MASPIN的SGC7901细胞株在G

期所占比例明显提高（70.3％ vs 55.6％），S期细胞减少（19.8% vs 34.9％，P0.05）。结论成功构建MASPIN/PCR2.1表达载体，过表达MASPIN可抑制SGC7901细胞株的增殖。

Abstract

Objective To construct a recombinant eukaryotic vector expressing maspin cDNA

and to explore the effect of maspin overexprssion on the proliferation of human gastric carcinoma cell line SGC7901. Methods The fragment of maspin gene was amplified by polymerase chain reaction (PCR). An eukaryotic vector expressing maspin (maspin/PCR2.1) was constructed with PCR2.1

and transfected into SGC7901 cells. The expression of maspin at mRNA and protein level was detected by RT

-PCR and Western blotting. The proliferation of SGC7901 cells was observed by MTT. Cell cycle was analyzed by flow cytometry. Results Recombinant plasmid maspin/PCR2.1 was constructed and transfected into SGC7901 successfully. The mRNA and protein levels of maspin were significantly higher in the maspin/PCR2.1 group (33.6±1.2

23.4±1.6) than that in the blank PCR2.1 (15.0±1.5

12.3±1.5) and the untreated group (13.7±2.0

12.0±1.3) (P0.05). The proliferation of SGC7901 cells was significantly lower (93.07%

81.97%

66.5%) in the maspin/PCR2.1 group at 24

72 h than that in the blank PCR2.1 group (95.98%

95.79%

95.59%) (P0.05). Compared with that in the blank PCR2.1 group

the G

phase proportion of the maspin/PCR2.1 group at 72 h was significantly higher (70.3% vs 55.6%

P0.05)

and the S phase proportion was lower (19.8% vs 34.9%

P0.05). Conclusion MASPIN overexpression can suppress the proliferation of gastric carcinoma cell line SGC7901.

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