Objective    To investigate the histomorphology

cellular biocompatibility in vitro and tensile force resistance property of fibrin-binding amniotic membrane (FBAM) and to explore its feasibility and superiority using as ocular surface graft.     Methods    After observing the morphology of FBAM under light microscope and scanning electron microscope

we did primary culture of corneal epithelial cells

taking monolayer fibrin-binding amniotic membrane as carrier

then observed cell growth overlay area. The elongation nature of FBAM was tested on  electronic universal testing machine by calculating elastic modulus

using bilayer amniotic membrane (BAM) and monolayer amniotic membrane (MAM) as controls.     Results    The fresh FBAM was integrated as a whole

with structurally complete fibrin and amniotic membrane as well as tight binding of fibrin to amniotic membrane. The reserved FBAM was structurally complete

brittler than the fresh FBAM

while amniotic membrane still bound tightly to the fibrin

but under microscope the fiber reticular formation was fairly fuzzy. Single layer of epithelial cells was formed on FBAM after about seven-day culture in vitro. Disparity of cell growth overlay area was significant (P0.05) from the 2nd day to the 7th day between experimental group and control group (denuded human amniotic membrane as control). The elastic modulus of FBAM was smaller than that of MAM and BAM (P0.01).     Conclusion    Amniotic membrane and fibrin can be integrated into FBAM. Amniotic membrane binds to fibrin tightly. The morphous of FBAM is regular. FBAM can be used as carrier for epithelial cells. FBAM possesses better biomechanical properties than MAM and BAM.