Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3

池余刚; 钟玲; 伍霞; 王勇

您当前的位置：

首页 >

文章列表页 >

Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3

更新时间：2026-01-09

- Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3
- Journal of Army Medical University Vol. 30, Issue 23, Pages: 2203-2206(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R73-361;R737.31
- Published：2008
- 稿件说明：
移动端阅览
池余刚,钟玲,伍霞,王勇.原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(23):2203-2206.

　CHI Yu-gang,ZHONG Ling,WU Xia,WANG Yong.Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(23):2203-2206.
池余刚,钟玲,伍霞,王勇.原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(23):2203-2206. DOI：

　CHI Yu-gang,ZHONG Ling,WU Xia,WANG Yong.Grape seed proanthocyanidin extract inhibits proliferation and promotes apoptosis of ovary cancer cell line SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(23):2203-2206. DOI：

摘要

目的探讨葡萄籽提取物原花青素对卵巢癌SKOV3细胞增殖和凋亡的影响和机制。方法体外培养的SKOV3细胞与25、50、100、200、400 μg/ml的葡萄籽提取物原花青素共孵育，分别在24、48、72 h时观察细胞形态学变化，MTT检测细胞增殖抑制率，流式细胞和TUNEL分析细胞凋亡，RT-PCR检测survivin在mRNA的表达情况

Western blot检测SKOV3细胞survivin在蛋白水平的表达。结果不同浓度(25、50、100、200、400 μg/ml)原花青素对SKOV3细胞作用48 h 的细胞增殖抑制率分别为48.67％、53.85％、67.03％、73.78％、77.39％。流式细胞和TUNEL检测经25 μg/ml原花青素处理24、48 h后SKOV3细胞凋亡率分别为31.98％、45.78％。葡萄籽原花青素还可以明显降低survivin的mRNA和蛋白水平的表达。这些作用均随原花青素浓度和作用时间的延长而增强。结论原花青素可能通过降低survivin的表达，在体外抑制SKOV3细胞增殖，并促进其凋亡。

Abstract

Objective To study the biological effects of grape seed proanthocyanidin extract (GSPE) on human ovary cancer cells and to explore the molecular mechanism. Methods Human ovary cancer cell line SKOV3 were co-cultured with GSPE solution of the terminal concentrations of 25

100

200

400 μg/ml respectively in 96-well plate. At 24

72 h after coculture

the following parameters were detected: cell growth curve

the inhibition rate of SKOV3 cells by MTT assay

DNA cycle by FCM

the apoptosis of SKOV3 by TUNEL and Annexin-V labeling method

the mRNA and protein expressions of survivin by semi-quantitative RT-PCR and Western blotting

respectively. Results GSPE dose-dependently inhibited the proliferation of the SKOV3 cells. Treated by GSPE

the progress of SKOV3 cells at S stage into G/M stage was inhibited. TUNEL showed that treated by 25 μg/ml GSPE for 24

48 h

the apoptotic rates of SKOV3 cells were 31.98%

45.78% respectively. Annexin-V showed that after incubation with 25 μg/ml GSPE for 24 h

the apoptotic rate was 14.68%. The survivin mRNA and protein expressions were both down-regulated. Conclusion GSPE inhibits the proliferation of malignant human ovary cancer cells and induces their apoptosis. Expression of survivin mRNA and protein may be related to cell growth inhibition and to the apoptosis mediated by GSPE in vitro.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of αB-crystallin on malignant behaviors of ovarian cancer cells

Role of PPARγ activation in cell growth inhibition and apoptosis inducement by ampelopsin in breast cancer cells

Silencing calcium-activated nucleotidase 1 inhibits proliferation and migration of clear cell renal cell carcinoma 769-P cells

Effects of Proteasome inhibitor bortezomib on apoptosis of murine alloreactive T cells in vitro

Related Author

张文君

刘斌

令狐华

Xu LIU

Xiaoli LI

Duanfang ZHOU

Bo CHEN

Yi SONG

Related Institution

Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology of Chongqing Universities, Chongqing Key Laboratory of Drug Metabolism, Department of Pharmacology, School of Pharmacy, Chongqing Medical University

Department of Nutrition and Food Safety, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Department of Clinic Nutrition, Banan District People's Hospital of Chongqing

Department of Urology, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

College of Life Sciences， Beijing University of Chinese Medicine

⁰