Establishment of classical eyeblink conditioned reflex model in conscious and unfixed guinea pigs and study of related acquisition rule

李蕊; 刘宏亮; 隋建峰; 范郑丽; 黄率帅; 万子兵; 赵红梅

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Establishment of classical eyeblink conditioned reflex model in conscious and unfixed guinea pigs and study of related acquisition rule

更新时间：2026-01-09

- Establishment of classical eyeblink conditioned reflex model in conscious and unfixed guinea pigs and study of related acquisition rule
- Journal of Army Medical University Vol. 31, Issue 5, Pages: 406-408(2009)
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  CLC： R322.91;R337.5;R338.61
- Published：2009
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李蕊,刘宏亮,隋建峰,范郑丽,黄率帅,万子兵,赵红梅.清醒无制动豚鼠经典眨眼条件反射的建立及其习得规律研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(05):406-408.

　LI Rui,LIU Hong-liang,SUI Jian-feng,FAN Zheng-li,HUANG Lu-shuai,WAN Zi-bing,ZHAO Hong-mei.Establishment of classical eyeblink conditioned reflex model in conscious and unfixed guinea pigs and study of related acquisition rule[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(05):406-408.
李蕊,刘宏亮,隋建峰,范郑丽,黄率帅,万子兵,赵红梅.清醒无制动豚鼠经典眨眼条件反射的建立及其习得规律研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(05):406-408. DOI：

　LI Rui,LIU Hong-liang,SUI Jian-feng,FAN Zheng-li,HUANG Lu-shuai,WAN Zi-bing,ZHAO Hong-mei.Establishment of classical eyeblink conditioned reflex model in conscious and unfixed guinea pigs and study of related acquisition rule[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(05):406-408. DOI：

摘要

目的以眼肌电记录法记录在清醒无制动豚鼠建立经典眨眼条件反射（conditioned reflex，CR）并分析其习得规律。方法采用金属电极埋植技术及简易“随动”气流给气刺激装置，以声音作为条件刺激(conditioned stimulus， CS)，气流作为非条件刺激(unconditioned stimulus， US)，以眼肌电判断其眨眼行为，在清醒无制动的豚鼠上进行经典眨眼条件反射的训练。结果可顺利于豚鼠的内眦采集并记录到眨眼肌电信号，动物可成功建立经典眨眼条件反射，其第4天，第7天，第10天的CR习得率分别可达31%、61.33%、82.67%，第15天，第20天的CR保持率可达到81.33%和82.33%。结论应用眼肌电记录技术及“随动”给气刺激方法可以在清醒无制动豚鼠建立经典眨眼条件反射实验。操作简单，方法可靠，成功率高，可用于对体型较大，不易固定动物的条件性眨眼行为研究。

Abstract

Objective To establish a model of classical eyeblink conditioned reflex (EBCR) in conscious and unfixed guinea pigs and to analyze the related acquisition rule. Methods With the aid of metal electrode embedding technique and simple follow-up air puff stimulus facility

conscious and unfixed guinea pigs were trained to performed EBCR with tone as conditioned stimulus (CS) and air puff as unconditioned stimulus (US). The eyeblink behavior was evaluated through electromyography (EMG) of musculi oculi. Results The eyeblink EMG signal of musculi oculi were successfully collected

and the classical eyeblink conditioning was obtained. The acquisition rate in the 4th

7th

and 10th day were 31%

61.3% and 82.7% respectively

and the maintain rate in the 15th and 20th day were 81.3% and 82.3%. Conclusion Conscious and unfixed guinea pigs can be trained to obtain EBCR with the techniques of musculi oculi EMG signal recording and follow-up air puff stimulating method. It is an easy

convenient

feasible

and reliable method with high achievement ratio. It could be applied to study conditioned eyeblink behavior of animals with big body type

or animals uneasy to be fixed.

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