Hypoxia-induced changes of JAK1/STAT1, 3 signal transduction pathway in PC12 cells

赵吉清; 林海; 吴强; 李云鹏; 董兆君

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Hypoxia-induced changes of JAK1/STAT1, 3 signal transduction pathway in PC12 cells

更新时间：2026-01-09

- Hypoxia-induced changes of JAK1/STAT1, 3 signal transduction pathway in PC12 cells
- Journal of Army Medical University Vol. 31, Issue 5, Pages: 391-393(2009)
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  CLC： R329.25;R394.2;R743.302
- Published：2009
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赵吉清,林海,吴强,李云鹏,董兆君.缺氧PC12细胞中JAK1/STAT1、STAT3介导的信号通路变化[J].陆军军医大学学报(原第三军医大学学报),2009,31(05):391-393.

　ZHAO Ji-qing,LIN Hai,WU Qiang,LI Yun-peng,DONG Zhao-jun.Hypoxia-induced changes of JAK1/STAT1, 3 signal transduction pathway in PC12 cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(05):391-393.
赵吉清,林海,吴强,李云鹏,董兆君.缺氧PC12细胞中JAK1/STAT1、STAT3介导的信号通路变化[J].陆军军医大学学报(原第三军医大学学报),2009,31(05):391-393. DOI：

　ZHAO Ji-qing,LIN Hai,WU Qiang,LI Yun-peng,DONG Zhao-jun.Hypoxia-induced changes of JAK1/STAT1, 3 signal transduction pathway in PC12 cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(05):391-393. DOI：

摘要

目的研究缺氧后PC12细胞蛋白酪氨酸激酶1/信号转导子与转录激活子1(janus kinase 1/signal transducer and activator of transcription 1

JAK1/STAT1)、STAT3 mRNA和蛋白表达变化。方法制备缺氧细胞模型。采用半定量RT-PCR技术和Western blot法检测缺氧PC12细胞的JAK1/STAT1、STAT3 mRNA和蛋白的表达水平，并对PCR产物进行测序。结果与对照组比较，缺氧2 h PC12细胞JAK1/STAT1、STAT3 mRNA和蛋白表达水平显著升高（P0.05），在缺氧6 h 达最高（P0.01），缺氧12 h的表达量降低（P0.05）。RT-PCR产物直接测序证实扩增产物片段与GenBank中相应序列相同。结论缺氧能增强PC12细胞中JAK1/STAT1、STAT3基因的表达，在缺氧所致脑损伤中可能发挥重要作用。

Abstract

Objective To study the effect of hypoxia on the expression of JAK1 and STAT1

3 in PC12 cell line. Methods PC12 cells were exposed to hypoxia and their mRNA and protein expression levels of JAK1 and STAT1

3 were detected by RT-PCR and Western blotting. The RT-PCR products were sequenced by Sanger’s double strand DNA sequence determination. Results The mRNA and protein expression levels of JAK1

STAT1

3 were increased in PC12 cells

reached the highest level at 6 h and decreased at 12 h

but always higher than that of control group. The sequences of amplification products by RT-PCR were the same as that in GenBank by sequencing. Conclusion Hypoxia reinforces the expressions of JAK1 and STAT1

3 in PC12 cells. JAK1/STAT1

3 possibly plays an important action in brain injury.

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