Objective    To develop a smoke inhalation unit simulating airtight cabin.     Methods    We designed a completed smoke inhalation unit composed of smoke generation cabinet

circulation pipe line and inhalation cabinet. The unit was verified with 42 SD rats inhaled with smoke generated from combustion of 9 nonmetal materials used in a  airtight cabin. The rats were randomly divided into experimental group and 5 inhalation groups

with 7 rats in each group. The concentrations of CO

O

2

and acid gases in the inhalation cabinet were analyzed. The activities and mortality of the rats within 7 d were recorded. COHb% of 21 rats in ten-minute inhalation groups was detected quickly after

exposure.     Results    The concentration of smoke increased with the time of combustion and kept constant on each time point. The degree of intoxication in rats increased with the time of inhalation

and COHb% of ten-minute inhalation groups showed good reproducibility.     Conclusion    Our developed unit can simulate the smoke generation and intoxication in airtight cabin and keep good reproducibility of animal injury.