Ursodeoxycholic acid, taurousrdeoxycholic acid or rifampicin induces expression of nuclear receptor FTF and membrane transporter MRP3 in HepG2 cells

柴进; 吴晓平; 夏锋; 何宇; 王槐志; 肖天利; 陈文生

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Ursodeoxycholic acid, taurousrdeoxycholic acid or rifampicin induces expression of nuclear receptor FTF and membrane transporter MRP3 in HepG2 cells

更新时间：2026-01-09

- Ursodeoxycholic acid, taurousrdeoxycholic acid or rifampicin induces expression of nuclear receptor FTF and membrane transporter MRP3 in HepG2 cells
- Journal of Army Medical University Vol. 31, Issue 2, Pages: 101-104(2009)
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  CLC： R341;R394.2;R657.302
- Published：2009
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柴进,吴晓平,夏锋,何宇,王槐志,肖天利,陈文生.熊脱氧胆酸和利福平诱导HepG2细胞核受体FTF和膜转运蛋白MRP3的表达[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):101-104.

　CHAI Jin,WU Xiao-ping,XIA Feng,HE Yu,WANG Huai-zhi,XIAO Tian-li,CHEN Wen-sheng.Ursodeoxycholic acid, taurousrdeoxycholic acid or rifampicin induces expression of nuclear receptor FTF and membrane transporter MRP3 in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):101-104.
柴进,吴晓平,夏锋,何宇,王槐志,肖天利,陈文生.熊脱氧胆酸和利福平诱导HepG2细胞核受体FTF和膜转运蛋白MRP3的表达[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):101-104. DOI：

　CHAI Jin,WU Xiao-ping,XIA Feng,HE Yu,WANG Huai-zhi,XIAO Tian-li,CHEN Wen-sheng.Ursodeoxycholic acid, taurousrdeoxycholic acid or rifampicin induces expression of nuclear receptor FTF and membrane transporter MRP3 in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):101-104. DOI：

摘要

目的体外细胞培养观察利胆药物熊脱氧胆酸（ursodeoxycholic acid

UDCA）及其衍生物牛磺熊脱氧胆酸（tauroursdeoxycholic acid

TUDCA）或利福平（rifampicin

Rif）对多耐药相关蛋白3（multidrug resistance-associate protein 3

MRP3）及其核受体FTF(fetoprotein transcription factor)表达的影响。方法用UDCA及其衍生物TUDCA或Rif分别刺激培养的肝癌细胞HepG2后，抽提HepG2的总RNA与细胞总膜蛋白、核蛋白，分别用Quantitative real-time PCR和Western blot检测MRP3和FTF mRNA与蛋白水平表达的变化。结果 UDCA及其衍生物TUDCA与Rif均可诱导HepG2细胞内核受体FTF表达增高，同时MRP3 mRNA和蛋白水平表达也相应增高。结论 UDCA及其衍生物TUDCA和Rif刺激肝癌细胞HepG2细胞膜转运蛋白MRP3表达上调可能与核受体FTF途径相关。

Abstract

Objective To observe the effect of ursodeoxycholic acid (UDCA)

and its derivative

tauroursdeoxycholic acid (TUDCA)

or rifampicin (Rif) on the expressions of membrane transporter

multidrug resistance-associate protein 3 (MRP3)

and its nuclear receptor

fetoprotein transcription factor (FTF) in HepG2 cells. Methods Both total RNA and total membrane or nuclear proteins were extracted from HepG2 cells after 48 h treatment with UDCA

TUDCA or Rif (100

100 or 10 μmol/L). The mRNA and protein expression levels of MRP3 and FTF were determined by quantitative real-time PCR and Western blot analysis. Results Both FTF and MRP3 expression was significantly induced either at mRNA or protein level by UDCA

TUDCA or Rif in HepG2 cells. Conclusion The up-regulation of hepatic MRP3 expression induced by UDCA

TUDCA or Rif may be associated with FTF pathway.

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