Effect of recombinant β<sub>3</sub> integrin on Dengue virus serotype 2 infection in CHO cells

张俊磊; 高娜; 陈炜; 陈宗涛; 徐小峰; 安静

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Effect of recombinant β₃ integrin on Dengue virus serotype 2 infection in CHO cells

更新时间：2026-01-09

- Effect of recombinant β₃ integrin on Dengue virus serotype 2 infection in CHO cells
- Journal of Army Medical University Vol. 31, Issue 2, Pages: 98-100(2009)
- 作者机构：
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  CLC： R373.33;R394-33;R512.802
- Published：2009
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张俊磊,高娜,陈炜,陈宗涛,徐小峰,安静.CHO细胞中稳定表达人整合素β3对登革2型病毒感染的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):98-100.

　ZHANG Jun-lei,GAO Na,CHEN Wei,CHEN Zong-tao,XU Xiao-feng,AN Jing.Effect of recombinant β3 integrin on Dengue virus serotype 2 infection in CHO cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):98-100.
张俊磊,高娜,陈炜,陈宗涛,徐小峰,安静.CHO细胞中稳定表达人整合素β3对登革2型病毒感染的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):98-100. DOI：

　ZHANG Jun-lei,GAO Na,CHEN Wei,CHEN Zong-tao,XU Xiao-feng,AN Jing.Effect of recombinant β3 integrin on Dengue virus serotype 2 infection in CHO cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):98-100. DOI：

摘要

目的检测CHO细胞中稳定表达人整合素β

对登革2型病毒(Dengue virus serotype 2

DV2)感染的影响。方法将表达人整合素β

的质粒转染CHO细胞，通过G418筛选、流式细胞学检测和间接免疫荧光实验，建立稳定表达整合素β

的CHO细胞株；利用该细胞株进行感染实验，检测人整合素β

稳定表达对DV2易感性的影响。结果共获得了C9、C10、F8和H6 4个高表达整合素β

的细胞克隆，其中克隆F8中整合素β

的表达水平最高，是对照组的1.89倍。感染实验结果显示，进入整合素β

高表达细胞株的病毒数量均有所增高，增高的比例与整合素β

的表达增高趋势基本一致，进入F8细胞株的病毒量最高，是对照细胞的2.63倍，说明整合素β

有助于DV2进入细胞。结论在CHO细胞中外源性表达整合素β

可以提高DV2的感染率。

Abstract

Objective To study the effect of recombinant β

integrin on Dengue virus serotype 2(DV2) infection of CHO cells. Methods Expression plasmid coding β

integrin was transfected into CHO cells using the lipofectamine reagent. Stable CHO cell lines expressing human β

integrin were selected in G4l8 and validated by flow cytometry and indirect immunofluorence. The stable cell lines were used to determine whether β

integrin expression facilitates DV2 infection. Results The stably transfected CHO variant c

ell lines C9

C10

F8 and H6 expressing human β

integrin were selected. The level of β

integrin expression in F8 was the highest

which was 1.89-fold of parental CHO cells. All the variant cell lines were more susceptible to DV2 infection

and F8 cell line was especially 2.63 times of parental CHO cells. Conclusion Recombinant β

integrin promotes DV2 infection of CHO cells.

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Related Institution

Department of Military Biosafety, College of Basic Medical Sciences, Army Medical University (Third Military Medical University)

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Effect of recombinant β3 integrin on Dengue virus serotype 2 infection in CHO cells

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Effect of recombinant β₃ integrin on Dengue virus serotype 2 infection in CHO cells