Activation of cultured mouse astrocytes by LPS and IFN-γ

熊加祥; 白云; 宋敏; 许雪青; 王艳艳; 杨晓亚

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Activation of cultured mouse astrocytes by LPS and IFN-γ

更新时间：2026-01-09

- Activation of cultured mouse astrocytes by LPS and IFN-γ
- Journal of Army Medical University Vol. 30, Issue 22, Pages: 2065-2068(2008)
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  CLC： R329.25;R338.1;R392.11
- Published：2008
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熊加祥,白云,宋敏,许雪青,王艳艳,杨晓亚.炎性介质脂多糖、γ干扰素活化星形胶质细胞效应[J].陆军军医大学学报(原第三军医大学学报),2008,30(22):2065-2068.

　XIONG Jia-xiang,BAI Yun,SONG Min,XU Xue-qing,WANG Yan-yan,YANG Xiao-ya.Activation of cultured mouse astrocytes by LPS and IFN-γ[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):2065-2068.
熊加祥,白云,宋敏,许雪青,王艳艳,杨晓亚.炎性介质脂多糖、γ干扰素活化星形胶质细胞效应[J].陆军军医大学学报(原第三军医大学学报),2008,30(22):2065-2068. DOI：

　XIONG Jia-xiang,BAI Yun,SONG Min,XU Xue-qing,WANG Yan-yan,YANG Xiao-ya.Activation of cultured mouse astrocytes by LPS and IFN-γ[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(22):2065-2068. DOI：

摘要

目的研究炎性介质脂多糖（LPS）、γ干扰素（IFN-γ）刺激离体星形胶质细胞的效应。方法 LPS、IFN-γ刺激离体培养纯化的小鼠星形胶质细胞，ELISA检测细胞分泌TNF-α，Griess法测定NO浓度，激光共聚焦扫描显微镜（CLSM）检测细胞内游离钙浓度（[Ca

]

i）。结果 LPS（500 ng/ml）、IFN-γ（100 U/ml）刺激星形胶质细胞24 h后， NO和TNF-α的分泌显著升高，联合应用有协同作用；延长IFN-γ作用时间，分泌量升高。CLSM检测LPS、IFN-γ刺激24 h后的星形胶质细胞[Ca

]

i的荧光值，显示荧光相对值比正常细胞明显升高；LPS、IFN-γ急性刺激正常培养细胞发现[Ca

]

i振荡上升；而作用于IFN-γ刺激24 h后的细胞，[Ca

]

i上升明显减缓。结论 LPS、IFN-γ可以活化星形胶质细胞，表现为[Ca

]

i升高，TNF-α和NO分泌上调。

Abstract

Objective To investigate the effects of inflammatory chemokine LPS and/or IFN-γ on astrocytes. Methods After LPS and IFN-γ applied to the purified cultured mouse astrocytes

TNF-α and nitric oxide (NO) secreted from astrocytes and intracellular calcium concentration ([Ca

]

i) were detected by ELISA

Griess and confocal laser scanning microscopy

respectively. Results Treatment with LPS (500 ng/ml) or IFN-γ (100 U/ml) for 24 h significantly increased the concentration of TNF-α and NO. Coadministration of LPS and IFN-γ showed a synergistic effect on the astrocytes. By using Ca

imaging

we found

that [Ca

]

i signal was significantly increased after LPS and/or IFN-γ treatment for 24 h. Also

acute treatment with LPS or IFN-γ could evoke Ca

oscillations. In contrast

after application of these stimuli over 24 h

the Ca

signal was increased more slowly. Conclusion Application of LPS and/or IFN-γ evoked TNF-α and NO secretion and [Ca

]

i elevation in cultured astrocytes

suggesting that astrocytes could be activated by LPS and IFN-γ.

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