Development of a nonencapsulated mutant of Streptococcus suis serotype 2 Chinese highly virulent strain 05ZYH33

胡丹; 王长军; 胡福泉; 王晶; 程功; 李明; 潘秀珍; 唐家琪

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Development of a nonencapsulated mutant of Streptococcus suis serotype 2 Chinese highly virulent strain 05ZYH33

更新时间：2026-01-09

- Development of a nonencapsulated mutant of Streptococcus suis serotype 2 Chinese highly virulent strain 05ZYH33
- Journal of Army Medical University Vol. 31, Issue 2, Pages: 93-97(2009)
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  CLC： R378.12;R394-33
- Published：2009
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胡丹,王长军,胡福泉,王晶,程功,李明,潘秀珍,唐家琪.猪链球菌2型中国强致病株05ZYH33荚膜缺陷菌株的构建[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):93-97.

　HU Dan,WANG Chang-jun,HU Fu-quan,WANG Jing,CHENG Gong,LI Ming,PAN Xiu-zhen,TANG Jia-qi.Development of a nonencapsulated mutant of Streptococcus suis serotype 2 Chinese highly virulent strain 05ZYH33[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):93-97.
胡丹,王长军,胡福泉,王晶,程功,李明,潘秀珍,唐家琪.猪链球菌2型中国强致病株05ZYH33荚膜缺陷菌株的构建[J].陆军军医大学学报(原第三军医大学学报),2009,31(02):93-97. DOI：

　HU Dan,WANG Chang-jun,HU Fu-quan,WANG Jing,CHENG Gong,LI Ming,PAN Xiu-zhen,TANG Jia-qi.Development of a nonencapsulated mutant of Streptococcus suis serotype 2 Chinese highly virulent strain 05ZYH33[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(02):93-97. DOI：

摘要

目的构建猪链球菌2型（Streptococcus suis serotype 2

S.suis 2）中国强致病株05ZYH33荚膜缺陷株。方法利用同源重组基因敲除方法获得S. suis 2强毒株05ZYH33荚膜合成基因cps2B敲除突变株，通过小鼠攻毒实验证实荚膜缺陷株对细菌毒力的影响。结果 PCR和Southern杂交结果均显示cps2B基因完全被壮观霉素抗性基因替代，表明基因敲除突变体构建成功。电镜结果证实突变体荚膜合成能力缺失，小鼠致病性实验结果显示突变体毒力基本丧失。结论成功构建05ZYH33荚膜缺陷株，提示菌体荚膜多糖成分对于猪链球菌2型侵袭和致病具有显著作用。

Abstract

Objective To construct a mutant of Streptococcus suis serotype 2 (S. suis 2) virulent strain 05ZYH33 by knocking out its cps2B gene which encoding capsular polysaccharide (CPS) synthase. Methods The cps2B gene in this 05ZYH33 strain was knocked out based on the principle of homologous recombination. PCR analysis and Southern hybridization were used to identify the knockout. Transmission electron microscopy was employed to observe the morphology of the established strain. The virulence of the deficient strain was tested on the murine model of infection. Results PCR analysis and Southern hybridization showed that cps2B was replaced completely by the Spc

cassette (encoding the gene resistant to spectinomycin). No capsu

lar material was observed on the surfaces of the mutant strain by transmission electron microscopy

suggesting that S. suis 2 mutant loss of ability in capsule synthesis. Furthermore

the nonencapsulated mutant was completely avirulent in murine model of infection. Conclusion The cps2B gene deletion mutant of 05ZYH33 was successfully constructed. The CPS synthase of S. suis 2 plays an essential role in the pathogenesis and invasiveness of S. suis 2 infection.

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