Comparative proteomic analysis on cerebral cortex mitochondria between ischemic and normal cerebral contex in rats

杨涌涛; 陈康宁; 贺伟峰; 黄河清; 刘国军; 周振华; 冉鸿

您当前的位置：

首页 >

文章列表页 >

Comparative proteomic analysis on cerebral cortex mitochondria between ischemic and normal cerebral contex in rats

更新时间：2026-01-09

- Comparative proteomic analysis on cerebral cortex mitochondria between ischemic and normal cerebral contex in rats
- Journal of Army Medical University Vol. 30, Issue 10, Pages: 964-968(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.27;R394-33;R743.31
- Published：2008
- 稿件说明：
移动端阅览
杨涌涛,陈康宁,贺伟峰,黄河清,刘国军,周振华,冉鸿.正常与脑缺血大鼠脑皮质线粒体的比较蛋白质组学研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):964-968.

　YANG Yong-tao,CHEN Kang-ning,HE Wei-feng,HUANG He-qing,LIU Guo-jun,ZHOU Zhen-hua,RAN Hong.Comparative proteomic analysis on cerebral cortex mitochondria between ischemic and normal cerebral contex in rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):964-968.
杨涌涛,陈康宁,贺伟峰,黄河清,刘国军,周振华,冉鸿.正常与脑缺血大鼠脑皮质线粒体的比较蛋白质组学研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):964-968. DOI：

　YANG Yong-tao,CHEN Kang-ning,HE Wei-feng,HUANG He-qing,LIU Guo-jun,ZHOU Zhen-hua,RAN Hong.Comparative proteomic analysis on cerebral cortex mitochondria between ischemic and normal cerebral contex in rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):964-968. DOI：

摘要

目的采用比较蛋白质组学方法观察正常与脑缺血大鼠脑皮质线粒体蛋白表达差异。方法 SD大鼠按随机数字表法分为正常组和缺血组，采用改进的栓线法制备大脑中动脉栓塞（middle cerebral antery occlusion

MCAO）模型，分离大鼠缺血及正常大脑皮层，纯化线粒体蛋白进行二维电泳和凝胶图像分析，对差异蛋白质用HPLC-Chip/MS纳流液质联用技术进行序列分析，经Spectrum Mill搜索NCBInr数据库后鉴定蛋白质。结果正常与脑缺血大鼠脑皮层线粒体蛋白表达存在显著差异，这些差异蛋白经鉴定，主要为TUC-4b、肌酸激酶同工酶、HS1结合蛋白、线粒体核糖体蛋白S27、异柠檬酸脱氢酶、丙酮酸脱氢酶、二氢硫辛酸脱氢酶、泛醌－细胞色素C还原酶、琥珀酸辅酶A还原酶、细胞色素氧化酶、F1-ATP beta链、热休克蛋白70、热休克蛋白60、脂酰辅酶A脱氢酶等差异线粒体蛋白。结论脑缺血可以导致皮层线粒体相关蛋白表达改变，提示其作用可能与线粒体能量代谢、凋亡有关。

Abstract

Objective To identify the proteins related to ischemia in the mitochondia of cerebral cortex. Methods Totally 7 adult male SD rats were inflicted by middle cerebral artery occlusion (MCAO) for 6 h with nylon monofilament. Another 7 rats served as normal control. Then cerebral cortex were collected and mitochondria were extracted and purified. After 2-dimensional electrophoresis and PDQuest for gel image analysis

the MS/MS of the peptides from the differential proteins digested by tripsin were analyzed by HPLC-Chip/MS and the original data were pre-processed by Spectrum Mill then search

ed in NCBInr database. Results Specific protein spots were identified as TUC-4b

CKB

HS1 binding protein

mitochondrial ribosomal protein S27

dihydrolipoamide dehydrogenase

malate dehydrogenase

isocitrate dehydrogenase(NAD+) alpha

dihydrolipoamide S-acetyltransferase

cytochrome c oxidase subunit Vla

succinate-coenzyme A ligase

ubiquinol-cytochrome c reductase core protein

ATP synthase beta subunit

heat shock 70×10

protein

heat shock 60×10

protein

acyl-coenzyme A dehydrogenase family. Conclusion Cerebebral ischemia affects the proteins related to cerebral cortex mitochondria

which suggests that these proteins may be correlated with the energy metabolism and apoptosis of mitochondria.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective effect of equol on mitochondrial damage induced by high glucose and fat in osteoblasts

Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism

Cinobufacini-induced apoptosis in human neuroblastoma SH-SY5Y cells

The apoptotic pathway of breast cancer multidrug resistant cells

Ovarian cancer cell apoptosis by high intensity electric pulses: mitochondrial mechanism

Related Author

李正宙

倪向敏

白倩

梁馨予

朱文艺

王建

刘景君

王晓明

Related Institution

Department of Frigid Zone Medicine， College of High Altitude Military Medicine, School of Nursing， Army Medical University （Third Military Medical University）

Key Laboratory of Extreme Environmental Medicine of Ministry of Education, School of Nursing， Army Medical University （Third Military Medical University）

Department of Clinical Nursing, School of Nursing， Army Medical University （Third Military Medical University）

Department of Neurosurgery， the Second Affiliated Hospital of Chongqing Medical University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

⁰