Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax

沈珠甫; 杨丽霞; 郭瑞威; 杨栋; 齐峰; 石燕昆

您当前的位置：

首页 >

文章列表页 >

Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax

更新时间：2026-01-09

- Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax
- Journal of Army Medical University Vol. 30, Issue 10, Pages: 957-959(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.123;R329.2;R965
- Published：2008
- 稿件说明：
移动端阅览
沈珠甫,杨丽霞,郭瑞威,杨栋,齐峰,石燕昆.神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):957-959.

　SHEN Zhu-fu,YANG Li-xia,GUO Rui-wei,YANG Dong,QI Feng,SHI Yan-kun.Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):957-959.
沈珠甫,杨丽霞,郭瑞威,杨栋,齐峰,石燕昆.神经酰胺对人内皮细胞凋亡及bax、bcl-2表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):957-959. DOI：

　SHEN Zhu-fu,YANG Li-xia,GUO Rui-wei,YANG Dong,QI Feng,SHI Yan-kun.Effects of ceramide on apoptosis of human endothelial cells and expressions of bcl-2 and bax[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):957-959. DOI：

摘要

目的观察外源性神经酰胺（C

-ceramide）对内皮细胞凋亡及相关基因bax和bcl-2表达变化的影响。方法原代培养人脐静脉血管内皮细胞，加药组加入终浓度分别为5、12.5、25、50 μmol/L的C

-ceramide，对照组加入体积分数为0.1%的无水乙醇。采用TUNEL法检测细胞凋亡

用RT-PCR、Western blot技术对bax mRNA、bcl-2 mRNA及蛋白表达进行分析。结果加药组内皮细胞的凋亡阳性率显著高于对照组(P0.05)，且具有时间和剂量依赖性；加药组bax mRNA及蛋白的表达较对照组显著升高（P0.05），bcl-2 mRNA及蛋白的表达较对照组则显著降低（P0.05）。结论外源性神经酰胺通过上调bax及下调bcl-2表达水平，改变bax/bcl-2值，从而诱导内皮细胞凋亡。

Abstract

Objective To observe the effect of ceramide on apoptosis of endothelial cells (EC) and determine the relationship between the ceramide-induced growth suppression and the expressions of bax and bcl-2. Methods Human umbilical endothelial cells were cultured in vitro and treated with 5

12.5

50 μmol/L C

-ceramide

the cells treated with absolute alcohol as control. TUNEL was employed to determine the apoptosis of the cultured EC. Bax and bcl-2 mRNA and protein levels of EC were detected by RT-PCR and Western blot. Results The number of apoptotic cells was significantly higher in ceramide-treated endothelial cells than that in the control group (P0

.05). Ceramide-induced EC apopotsis was in time- and dose-dependent manner. Compared with the control cells

the expressions of bax mRNA and protein in EC treated with ceramide significantly increased (P0.05)

while the expressions of bcl-2 mRNA and protein significantly depressed (P0.05). Conclusion Ceramide may induce apoptosis of endothelial cells by down-regulating bcl-2 and up-regulating bax.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Reversal of apoptosis resistance of adriamycin-resistant human bladder carcinoma cell line BIU-87/ADM by tetrandrine and its possible mechanism

Effect of Toxoplasma gondii RH strain on apoptosis of mouse placental trophoblastic cells

Lycium barbarum polysaccharide inhibits apoptosis of intestinal epithelial cells through mitochondrial pathway in mice with radiation-induced intestinal injury

Effects of esmolol on cardiomyocyte apoptosis in septic rats

Edaravone down-regulates Bax but up-regulates Bcl-2 in rabbits after intracerebral hemorrhage

Related Author

黄玉清

李永生

刘锦裕

葛璞

车艺

林玲

黄开顺

彭净

Related Institution

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Immunology, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Laboratory Animal Science， College of Basic Medical Sciences, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Pharmaceutical Analysis, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Spine Surgery， the Second Affiliated Hospital of Chongqing Medical University

⁰