Objective    To investigate the effect of interleukin-10 (IL-10) on high glucose induced epithelial to mesenchymal transition in human renal tubular cells.     Methods    Human renal proximal tubular cell line HK-2 cells were divided randomly into 5 groups. Group 1 served as control

and its HK-2 cells were incubated with 5.5 mmol/L D-glucose．The cells of group 2 were cultured with 30 mmol/L D-glucose. For the cells of groups 3

4 and 5

1

5 or 25 ng/ml IL-10 was added in their culture media beside the presupplement of 30 mmol/L D-glucose. In 48 h after culture

RT-PCR was used to detect the mRNA expression of connective tissue growth factor (CTGF)

immunocytochemical assay and Western blot analysis were used to measure the expression of alpha-smooth muscle actin (α-SMA)

and ELISA was used to determine the secretion of fibronectin (FN) in the supernatant.     Results    Compared with group 2

CTGF

α-SMA and FN were all lower in groups 3

4 and 5 (P0.05).     Conclusion    IL-10 can inhibit the renal tubular epithelial to mesenchymal transition induced by high glucose concentration.