Objective    To observe the effects of genistein on the proliferation and invasion of the cultured human keloid fibroblasts and explore the mechanism of its anti-fibrosis.     Methods    The human keloid fibroblasts were cultured and stimulated with 25

50

100  mol/L genistein

respectively. Their proliferation was measured by MTT. The collagen synthesis was assessed by

3

H-proline incorporation method. The invasion of human keloid fibroblasts was detected in Transwell chemotaxis chamber model.     Results    Genistein markedly inhibited the proliferation and collagen synthesis of the cultured keloid fibroblasts and the inhibitory effects were increased with the increasing concentration of genistein. Genistein also weakened the invasion of

keloid fibroblasts.     Conclusion    Genistein has the ability of anti-fibrosis and inhibits the invasion of human keloid fibroblasts in vitro

indicating genistein may be an effective drug for pathological scar or fibrosis disease.