Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H<sub>22</sub>

李静; 沈宜; 杨麟; 段红; 郑维平

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Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H₂₂

更新时间：2026-01-09

- Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H₂₂
- Journal of Army Medical University Vol. 30, Issue 19, Pages: 1836-1838(2008)
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  CLC： R392.32;R73-362;R735.7
- Published：2008
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李静,沈宜,杨麟,段红,郑维平.小鼠肝癌细胞(H22)源外泌体的体内抗肝癌作用研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(19):1836-1838.

　LI Jing,SHEN Yi,YANG Lin,DUAN Hong,ZHENG Wei-ping.Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(19):1836-1838.
李静,沈宜,杨麟,段红,郑维平.小鼠肝癌细胞(H22)源外泌体的体内抗肝癌作用研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(19):1836-1838. DOI：

　LI Jing,SHEN Yi,YANG Lin,DUAN Hong,ZHENG Wei-ping.Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(19):1836-1838. DOI：

摘要

目的在荷瘤动物模型观察小鼠肝癌细胞（H

）来源的外泌体(exosomes)激发宿主抗肝癌免疫应答的效应。方法用自行分离纯化的小鼠肝癌细胞H

源外泌体免疫小鼠，然后给小鼠皮下注射H

肿瘤细胞，观察肿瘤的生长状况。用MTT法检测小鼠脾细胞增殖情况和细胞毒活性;用免疫组织化学法检测肿瘤组织中CD4

、CD8

T淋巴细胞浸润情况。结果该外泌体使肿瘤出现时间延迟，肿瘤生长缓慢，小鼠生存率提高。促进脾淋巴细胞增殖并增强其细胞毒活性，促进CD4

和CD8

T细胞活化。结论小鼠肝癌细胞（H

）源外泌体能诱导抗肿瘤应答，有效地诱导特异性的杀伤肿瘤细胞活性，有针对亲本细胞的免疫保护作用。

Abstract

Objective To investigate the induction of antitumor response and its immune mechanism of H

cell-derived exosomes. Methods Fifteen BALB/c mice were immunized by H

cell-derived exosomes three times at weekly intervals

then inoculated with the wild-type H

. Another 15 BALB/c mice that were injected with PBS were used as controls. In the 80-day observation period

the tumor growth and survival time were observed. MTT was used to determine lymphocyte proliferation and specific cytotoxicity of mice splenic cells. Immunohistochemical staining of CD4

and CD8

was performed for lymphocyte infiltr

ation in tumor tissues. Results Immunization with exosomes derived from H

delayed the tumor growth and promoted the survival rate of the mice bearing the tumor

and also induced lymphocyte proliferation and triggered strong specific cytotoxic response of spleen lymphocytes and activated CD4

and CD8

T cells. Conclusion Vaccination of H

cell-derived exosomes is effective in inducing the protective immunity. Furthermore

those exosomes have strong antitumor activities.

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Related Institution

Department of Hepatobiliary， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Digestive Disease Center， Chongqing Hygeia Hospital

Institute of Hepatobiliary Surgery of PLA，First Affiliated Hospital， Army Medical University （Third Military Medical University）

No.953，Xigaze， Tibet Autonomous Region

Department of Biochemistry and Molecular Biology

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Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22

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Abstract

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Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H₂₂