Effects of B-type natriuretic peptides on expression of monocyte chemoattractant protein-1 in rat peritoneal macrophages in vitro

郜攀; 黄岚; 郭瑞威; 钱德慧

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Effects of B-type natriuretic peptides on expression of monocyte chemoattractant protein-1 in rat peritoneal macrophages in vitro

更新时间：2026-01-09

- Effects of B-type natriuretic peptides on expression of monocyte chemoattractant protein-1 in rat peritoneal macrophages in vitro
- Journal of Army Medical University Vol. 30, Issue 15, Pages: 1452-1455(2008)
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  CLC： R392.12;R392.3;R972.1
- Published：2008
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郜攀,黄岚,郭瑞威,钱德慧.B型利钠肽对小鼠腹腔巨噬细胞MCP-1蛋白表达的双向调节作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1452-1455.

　GAO Pan,HUANG Lan,GUO Rui-wei,QIAN De-hui.Effects of B-type natriuretic peptides on expression of monocyte chemoattractant protein-1 in rat peritoneal macrophages in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1452-1455.
郜攀,黄岚,郭瑞威,钱德慧.B型利钠肽对小鼠腹腔巨噬细胞MCP-1蛋白表达的双向调节作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1452-1455. DOI：

　GAO Pan,HUANG Lan,GUO Rui-wei,QIAN De-hui.Effects of B-type natriuretic peptides on expression of monocyte chemoattractant protein-1 in rat peritoneal macrophages in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1452-1455. DOI：

摘要

目的观察B型利钠肽（B type natriuretic peptides，BNP）在小鼠腹腔巨噬细胞内介导单核细胞趋化蛋白-1（monocyte chemoattractant protein-1，MCP-1）蛋白的表达，探讨BNP在小鼠腹腔巨噬细胞中调节炎症反应的作用。方法原代培养小鼠腹腔巨噬细胞，分别予不同剂量的BNP、BNP+GC-A受体抑制剂（HS-142-1）、BNP+肿瘤坏死因子-α（TNF-α）+HS-142-1预处理细胞，设立空白对照组。提取培养细胞蛋白，采用Western blot法检测细胞内MCP-1蛋白表达。结果在小鼠巨噬细胞内BNP能够促进MCP-1蛋白表达，该作用可被BNP抑制剂HS-142-1所抑制；且TNF-α在巨噬细胞中促进MCP-1分泌的作用可以被BNP抑制。结论 BNP能够促进巨噬细胞释放MCP-1，促进炎症反应的发生；同时BNP能够抑制TNF-α促进MCP-1蛋白分泌的作用，提示BNP具有炎症反应的双向调节作用。

Abstract

Objective To investigate the effects of B-type natriuretic peptides (BNP) on expression of monocyte chemoattractant protein-1(MCP-1) in rat peritoneal macrophages and to identify the inflammation-mediated effects of BNP in macrophages. Methods Peritoneal macrophages of primary culture were treated with BNP

BNP+HS-142-1

or BNP+TNF-α+HS-142-1. The protein expression of MCP-1 was measured by Western blot. Results BNP enhanced the MCP-1 protein expression in macrophages

and this effect could be abrogated by HS-142-1. In addition

BNP could inhibit TNF-α induced MCP-1 expression. Conclusion BNP can induce the MCP-1 protein expression in macrophages

suggesting BNP has a pro-inflammatory effect. However

BNP also can inhibit TNF-α induced MCP-1. These findings suggest that the effect of inflammation-mediated by BNP is biphasic though the mechanism is still unclear.

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