Objective    To observe the relationship between zonula occludens protein subunit 1 (ZO-1) and the regulatory effect of vasodilator stimulated phosphoprotein (VASP) on the intestinal barrier function in rats of hemorrhagic shock (HS).     Methods    The intestinal barrier function was reflected by the hematoplasma D-lactic acid content

and the mucous membrane of small intestine from HS rats at different time after shock (1

2 and 4 h) were adopted to determine the expressions of VASP

phospho-VASP and ZO-1. cAMP

the agonist of VASP phosphorylation

was employed to observe the change of the above mentioned molecules and the intestinal barrier function following HS.     Results    (1) VASP expression of small intestine mucous membrane was decreased significantly after HS (P0.01)

phospho-VASP was mildly expressed in normal control group

and exhibited a time-dependent change following HS

increased at 1 h (P0.01) and decreased to a low level after 2 h (P0.01). ZO-1 expression was decreased following HS

significantly after 2 h (P0.01). The hematoplasma D-lactic acid content did not change much 1 h after shock

while significantly increased after 2 h (P0.01). (2) cAMP increased the expressions of VASP

phospho-VASP and ZO-1

and decreased hematoplasma D-lactic acid content at 2 h shock.     Conclusion    ZO-1 may be involved in the regulatory effect of VASP on intestinal barrier function in HS rats.