Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721

张丽杰; 鹿刚; 张引娟; 刘刚叁; 单保恩

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Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721

更新时间：2026-01-09

- Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721
- Journal of Army Medical University Vol. 30, Issue 15, Pages: 1448-1451(2008)
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  CLC： R285.5;R730.23;R735.7
- Published：2008
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张丽杰,鹿刚,张引娟,刘刚叁,单保恩.香加皮提取物杠柳苷抑制SMMC-7721细胞Stat3信号通路诱导细胞凋亡的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1448-1451.

　ZHANG Li-jie,LU Gang,ZHANG Yin-juan,LIU Gang-san,SHAN Bao-en.Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1448-1451.
张丽杰,鹿刚,张引娟,刘刚叁,单保恩.香加皮提取物杠柳苷抑制SMMC-7721细胞Stat3信号通路诱导细胞凋亡的研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1448-1451. DOI：

　ZHANG Li-jie,LU Gang,ZHANG Yin-juan,LIU Gang-san,SHAN Bao-en.Periplocin of cortex periplocae inhibits Stat3 signaling and induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1448-1451. DOI：

摘要

目的研究香加皮提取物杠柳苷对Stat3信号通路的影响，及其诱导细胞凋亡的分子机制。方法 MTT比色法检测人肝癌细胞SMMC-7721增殖活性，流式细胞术分析肿瘤细胞的周期变化和凋亡，Western blot检测细胞内Stat3蛋白表达，RT-PCR检测细胞Mcl-1、Survivin和XIAP mRNA的表达。结果杠柳苷可明显抑制SMMC-7721细胞的增殖，诱导SMMC-7721细胞凋亡，并使细胞周期停滞在G

/M期。杠柳苷作用后，细胞核内Stat3量降低，而细胞质中的量未见明显改变，细胞内Mcl-1、Survivin和XIAP基因的表达明显降低，并呈时间依赖性。结论香加皮提取物杠柳苷通过抑制Stat3信号转导通路和Mcl-1、Survivin和XIAP mRNA的表达，进而抑制SMMC-7721细胞的增殖，诱导其凋亡。

Abstract

Objective To investigate the effect of periplocin of cortex periplocae (CPP) on Stat3 signaling and its probable molecular mechanism of inducing apoptosis and anti-tumor activity. Methods Cell proliferation was detected by MTT. Cell apoptosis and cell cycle were investigated by flow cytometry. Expression of Stat3 protein in SMMC-7721 cells was analyzed by Western blot. Mcl-1

Survivin and XIAP mRNA expressions were measured by RT-PCR. Results CPP inhibited the proliferation of SMMC-7721 cells significantly

induced their apoptosis and arrested their cell cycle at G

/M phase. Decreased expressi

on of Stat3 protein in the cell nucleus was observed after CPP treatment

but no significant changes were found in cytoplasma. Mcl-1

Survivin and XIAP mRNA expression levels were decreased in a time-dependent manner. Conclusion CPP inhibits cell proliferation and induces apoptosis by inhibiting Stat3 signal transduction in human hepatocellular carcinoma cell line SMMC-7721.

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