S100 calcium-binding protein A2 upregulates the activity of Wnt/β-catenin signaling pathway

赖天霞; 苗静琨; 左国伟; 何焕玲; 李星星; 卫佳; 吴丽美; 寇小琴; 何通川; 周兰

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S100 calcium-binding protein A2 upregulates the activity of Wnt/β-catenin signaling pathway

更新时间：2026-01-09

- S100 calcium-binding protein A2 upregulates the activity of Wnt/β-catenin signaling pathway
- Journal of Army Medical University Vol. 30, Issue 16, Pages: 1545-1548(2008)
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  CLC： Q513;R394.3;R730.23
- Published：2008
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赖天霞,苗静琨,左国伟,何焕玲,李星星,卫佳,吴丽美,寇小琴,何通川,周兰.钙结合蛋白S100A2对Wnt/β-catenin信号途径活性的上调作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(16):1545-1548.

　LAI Tian-xia,MIAO Jing-kun,ZUO Guo-wei,HE Huan-ling,LI Xing-xing,WEI Jia,WU Li-mei,KOU Xiao-qin,HE Tong-chuan,ZHOU Lan.S100 calcium-binding protein A2 upregulates the activity of Wnt/β-catenin signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(16):1545-1548.
赖天霞,苗静琨,左国伟,何焕玲,李星星,卫佳,吴丽美,寇小琴,何通川,周兰.钙结合蛋白S100A2对Wnt/β-catenin信号途径活性的上调作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(16):1545-1548. DOI：

　LAI Tian-xia,MIAO Jing-kun,ZUO Guo-wei,HE Huan-ling,LI Xing-xing,WEI Jia,WU Li-mei,KOU Xiao-qin,HE Tong-chuan,ZHOU Lan.S100 calcium-binding protein A2 upregulates the activity of Wnt/β-catenin signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(16):1545-1548. DOI：

摘要

目的研究钙结合蛋白S100A2对Wnt/β-catenin信号途径活性的影响，并探讨其可能的机制。方法原核诱导表达GST-hS100A2，经纯化后加入骨肉瘤细胞株MG63和人结肠癌细胞株HCT116的培养液中，Western blot检测细胞中β-catenin含量的变化；荧光素酶活性分析法检测S100A2对HEK293细胞中β-catenin/TCF4活性的影响；以表达GSK-3β、DVL、Axin的相应质粒分别转染HEK293细胞，GST-Pulldown/Western blot实验检测S100A2与这些蛋白质和β-catenin之间的相互作用。结果 S100A2使MG63和HCT116细胞中β-catenin含量增加、β-catenin/TCF4活性增强；S100A2分别与β-catenin和GSK-3β之间存在相互作用，而与DVL和Axin之间则未发现相互作用。结论 S100A2可以上调Wnt/β-catenin信号途径的活性，其机制可能涉及S100A2与β-catenin和GSK-3β之间的相互作用。

Abstract

Objective To investigate the effects of calcium-binding protein S100 A2 on Wnt/β-catenin signaling pathway and its mechanisms. Methods Human osteosarcoma cell line MG63 and human colon cancer cell line HCT116 were treated with recombinant fusion protein GST-hS100A2 that was expressed in E.coli and purified. β-catenin was quantified by Western blot and the effects of S100A2 on the β-catenin/TCF4 activity was analyzed by luciferase activity assay. The interactions between S100A2 and β-catenin/GSK-3β/DVL/Axin were detected by GST-Pulldown and Western blot. Results The β-catenin level in MG63 and HCT cells treated with GST-hS100A2 increased significantly as compared with that in the GST control group (P0.01). The luciferase activity in HEK 293 cells transfected with pTOP-Luc and then treated with GST-hS100A2 increased by 12.3 fold as compared with that in the GST control group (P0.01). The interactions between GST-hS100A2 and β-catenin/GSK-3β were verified by GST-Pulldown/Western blot

and no interaction was found between GST-hS100A2 and Axin/DVL. Conclusion S100A2 can upregulate the activity of Wnt/β-catenin signaling pathway and its mechanism may involve in the interactions between S100A2 and β-catenin/GSK-3β.

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