NGF participates in the airway inflammation of asthmatic mice

郑晓晶; 张桂蓉

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NGF participates in the airway inflammation of asthmatic mice

更新时间：2026-01-09

- NGF participates in the airway inflammation of asthmatic mice
- Journal of Army Medical University Vol. 30, Issue 11, Pages: 1058-1061(2008)
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  CLC： R392-33;R392.3;R562.25
- Published：2008
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郑晓晶,张桂蓉.NGF参与支气管哮喘大鼠气道炎症的发生及作用机制[J].陆军军医大学学报(原第三军医大学学报),2008,30(11):1058-1061.

　ZHENG Xiao-jing,ZHANG Gui-rong.NGF participates in the airway inflammation of asthmatic mice[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):1058-1061.
郑晓晶,张桂蓉.NGF参与支气管哮喘大鼠气道炎症的发生及作用机制[J].陆军军医大学学报(原第三军医大学学报),2008,30(11):1058-1061. DOI：

　ZHENG Xiao-jing,ZHANG Gui-rong.NGF participates in the airway inflammation of asthmatic mice[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):1058-1061. DOI：

摘要

目的探讨神经生长因子（nerve growth factor

NGF）对支气管哮喘大鼠气道炎症的影响及作用机制。方法 24只雄性SD大鼠分为正常对照组（A组）、哮喘组（B组）和抗NGF组（C组），每组8只。测定各组支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）总细胞及细胞分类计数；HE染色观察气道病理改变；RT-PCR和ELISA法检测各组大鼠白细胞介素-4（interleukin-4，IL-4）、干扰素-γ（interferon-γ，IFN-γ）、NGF mRNA和蛋白表达水平。结果 B组与A组比较，BALF中细胞总数及细胞分类计数增多（P0.05)；IL-4、NGF mRNA和蛋白表达增加（P0.01)；而IFN-γ mRNA和蛋白表达减少（P0.01)。C组与B组比较，BALF中细胞总数及细胞分类计数减少（P0.05)；IL-4、NGF mRNA和蛋白表达减弱（P0.01)；而IFN-γ mRNA和蛋白表达水平无显著差异。B组BALF中NGF含量与细胞总数、嗜酸性粒细胞、淋巴细胞呈正相关（r=0.833、0.944、0.949，P＜0.05)；与IL-4含量呈正相关（r=0.944，P0.01)，与IFN-γ含量不相关（r=0.122，P0.05）；与肺组织中IL-4 mRNA表达水平呈正相关（r=0.848，P0.05)，与IFN-γ mRNA表达不相关（r=0.611，P0.05）。结论 NGF与哮喘大鼠气道炎症密切相关，它可以通过使Th2型细胞因子分泌亢进参与哮喘的发生。

Abstract

Objective To investigate the effects and mechanism of nerve growth factor (NGF) on the airway inflammation of asthmatic mice. Methods Male SD rats (n=24) were randomly divided into the control group (group A)

the asthmatic group (group B)

the anti-NGF group (group C)

8 mice in each group. The total cell count and classified count of cells in the bronchoalveolar lavage fluid (BALF) were performed.The pathological changes of the lung tissues were detected by HE staining. The concentrations of IL-4

IFN-γ and NGF in BALF were measured by ELISA. The mRNA expression of IL-4

IFN-γ and NGF in the lung tissues were detected by RT-PCR. Results Compared with the group A

not only the number of the total cells and classified cells in BALF significantly increased (P0.05) in the group B

but also the mRNA and protein levels of IL-4 and NGF (P0.01)

but the mRNA and protein levels of IFN-γ significantly decreased (P0.01). Compared with the group B

the number of the total cells and classified cells in BALF significantly decreased (P0.05) in the group C

while the mRNA and protein levels of IL-4 and NGF significantly decreased (P0.01)

but the mRNA and protein levels of IFN-γ showed no significant difference. The NGF levels in BALF of the group B were positively correlated with the number of total cells

eosinophils and lymphocytes in BALF (r=0.833

0.944

0.949

P0.05)

the mRNA and protein levels of IL-4 in BALF (r=0.848

0.944 respectively

P0.01)

but not correlated with the mRNA and protein levels of IFN-γ in BALF(r=0.611

0.122 respectively

P0.05). Conclusion NGF may play a key role in the formation of airway inflammation in the asthmatic rats by promoting the secretion of Th2 cytokines.

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Department of Respiratory Medicine, Key Laboratory of Child Development and Disorders of Ministry of Education, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University

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