RNA interference silencing nucleophosmin 1 inhibits NPM1 gene expression in K562 cells

何鹏; 骆展鹏; 张伶; 杨松; 钟晓明; 高玉洁

您当前的位置：

首页 >

文章列表页 >

RNA interference silencing nucleophosmin 1 inhibits NPM1 gene expression in K562 cells

更新时间：2026-01-09

- RNA interference silencing nucleophosmin 1 inhibits NPM1 gene expression in K562 cells
- Journal of Army Medical University Vol. 30, Issue 10, Pages: 938-941(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.3;R73-362;R733.7
- Published：2008
- 稿件说明：
移动端阅览
何鹏,骆展鹏,张伶,杨松,钟晓明,高玉洁.RNAi重组体抑制白血病K562细胞系中NPM1基因的表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):938-941.

　HE Peng,LUO Zhan-peng,ZHANG Ling,YANG Song,ZHONG Xiao-ming,GAO Yu-jie.RNA interference silencing nucleophosmin 1 inhibits NPM1 gene expression in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):938-941.
何鹏,骆展鹏,张伶,杨松,钟晓明,高玉洁.RNAi重组体抑制白血病K562细胞系中NPM1基因的表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):938-941. DOI：

　HE Peng,LUO Zhan-peng,ZHANG Ling,YANG Song,ZHONG Xiao-ming,GAO Yu-jie.RNA interference silencing nucleophosmin 1 inhibits NPM1 gene expression in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):938-941. DOI：

摘要

目的构建核仁磷酸蛋白1 (nucleophosmin1，NPM1)特异性的RNAi真核表达载体，观察对白血病K562细胞系NPM1表达的抑制作用。方法采用RT-PCR检测白血病细胞系(THP1和K562)和急性髓系白血病（AML）细胞NPM1 mRNA水平。设计合成2条针对NPM1基因并具有小发夹结构的DNA序列，经退火形成互补双链，再克隆至载体pGenSil-1中构建含NPM1的RNAi重组体，经酶切及测序鉴定后通过脂质体转染K562细胞，同时设立pGenSil-1转染组和pGenSil-1/neg转染组。采用RT-PCR检测各组转染细胞中NPM1的mRNA水平。结果白血病细胞系和AML细胞均高表达NPM1 mRNA。针对人NPM1基因的RNAi重组体构建成功，并能够稳定转染K562细胞，导致白血病细胞NPM1 mRNA相对表达量下降64％，而pGenSil-1转染组和pGenSil-1/neg转染组未能下调NPM1表达。结论白血病细胞高表达NPM1基因，其mRNA表达水平能够被靶向NPM1的RNAi重组体特异性地下调。

Abstract

Objective To construct eukaryotic expression vector of RNAi specific to nucleophosmin1 (NPM1) and investigate the effect of recombinant plasmid on NPM1 expression in K562 cells. Methods The expression level of NPM1 mRNA was detected by RT-PCR in leukemia cell lines (THP1 and K562)

bone marrow or blood samples from 5 patients with acute myelogenous leukemia (AML) and from 5 healthy subjects. A short hairpin RNA targeting NPM1 was synthesized and inserted into the plasmid pGenSil-1. After the recombinant plasmid was identified

the correct plasmid was transfected into K562 cells

then the expression level of NPM1 mRNA was detected by RT-PCR. Results NPM1 mRNA levels were higher in two leukemia cell lines and all AML samples than those in normal cells. The RNAi recombinant plasmid was constructed successfully. The NPM1 mRNA levels decreased by 64% in K562 cells after the recombinant plasmid transfection

but those were not inhibited in K562 cells transfected with empty plasmid and untransfected K562 cells. Conclusion NPM1 mRNA is overexpressed in leukemia cells. The RNAi recombinant targeting NPM1 suppresses the expression of NPM1 mRNA.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Construction of recombinant adenovirus vector of Livin siRNA and its effects on proliferation, apoptosis and drug resistance of K562 cells

Effect of RNA-silenced survivin gene on sensitivity of K562 cells to adriamycin

Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells

Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines

Impact of molecular heterogeneity of NPM1 mutations on prognosis of acute myeloid leukemia: a clinical report of 86 cases

Related Author

郭玥馨

毛成刚

罗庆

郑改焕

徐酉华

陈琳

徐酉华

温贤浩

Related Institution

the First Clinical Medical College of Lanzhou University, the First Hospital of Lanzhou University

Department of Hematology, the First Hospital of Lanzhou University

⁰