Objective    To observe the effect of keratinocyte growth factor (KGF) on PCNA

ER and PR expressions of cultured endometrial epithelial cells (EECs) from the patients with anovulatory dysfunctional uterine bleeding (ADUB).      Methods    EECs from the patients with ADUB were separated and cultured with KGF of different concentration

with the normal EECs as control. Proliferative cell nuclear antigen (PCNA)

estrogen receptor (ER)

and progesterone receptor (PR) were detected by immunocytochemistry. The mRNA expressions of ER and PR were determined by RT-PCR.     Results    PCNA was only expressed within nucleus. ER and PR proteins were mainly expressed within nucleus

and some also expressed in plasma. KGF (50 ng/ml) increased PCNA

ER and PR expressions in both ADUB and normal EECs (P0.05). KGF (50 ng/ml) increased the mRNA expressions of ER and PR in both ADUB and normal EECs (P0.05). The increment of PCNA and ER expressions in ADUB group was the same as that in normal group (P0.05)

but the increment of PR expressions in ADUB group was more than that in normal group (P0.05).     Conclusion    KGF can increase PCNA

ER and PR expressions in cultured ADUB and normal EECs

but the increment in the two groups is significantly different

indicating that EECs from ADUB endometrium and normal endometrium react to the stimulation of KGF in different ways.