Objective    To prepare and select monoclonal antibodies (McAbs) with high specificity  against Vibrio cholera O1 Serotype Inaba and to provide a potent medical tool for the prevention and diagnosis of cholera.     Methods    BALB/c mice were immunized with Vibrio choler

ae O1 Serotype Inaba. Hybridoma cell lines secreting monoclonal antibodies against Vibrio cholerae O1 Serotype Inaba were established after cell fusion of mouse splenic cells and SP2/0. Both of these McAbs were characterized for their antibody titre in the culture supernatant as well as ascites

class

sub-class and affinity analysis. The antigen specificity and strain cross reactivity for McAb were evaluated and confirmed by indirect enzyme-linked immunosorbent assay (ELISA) and Western blot. The antigen epitope identifying of McAb were observed by ELISA additive assay

indirect ELISA with LPS of cholerae O1 Serotype Inaba and Western blot.     Results    Totally 386 positive monoclonal cell lines were produced; four of them showing high specificity for Vibrio cholerae O1 Serotype Inaba. As to the immunoglobulin subclass of McAb

two were IgG

1

others were IgG

2a

and IgG

2b

respectively. Indirect proof from ELISA and Western blot showed that the McAbs reacted specifically with Vibrio cholerae O1 Serotype Inaba.  Results from ELISA additive assay indicated that these McAbs recognized different epitopes. The reaction with LPS indicated that two McAbs were targeted to the LPS of cholerae O1 Serotype Inaba

while others were targeted to the non-LPS sites of cholerae O1 Serotype Inaba.      Conclusion    Four highly specific McAbs against Vibrio cholerae O1 Serotype Inaba were obtained successfully. The antigen epitope by combined experimental site was located initially. It provides a fast and exact tool on the early diagnosis and prevention of Vibrio cholerae O1 Serotype Inaba.