Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin

李强; 程湘; 孙守勋; 刘莹

您当前的位置：

首页 >

文章列表页 >

Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin

更新时间：2026-01-09

- Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin
- Journal of Army Medical University Vol. 30, Issue 15, Pages: 1438-1440(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-354;R737.9;R979.11
- Published：2008
- 稿件说明：
移动端阅览
李强,程湘,孙守勋,刘莹.乳腺癌细胞过表达iASPP对顺铂作用的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1438-1440.

　LI Qiang,CHENG Xiang,SUN Shou-xun,LIU Ying.Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1438-1440.
李强,程湘,孙守勋,刘莹.乳腺癌细胞过表达iASPP对顺铂作用的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1438-1440. DOI：

　LI Qiang,CHENG Xiang,SUN Shou-xun,LIU Ying.Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1438-1440. DOI：

摘要

目的构建过表达iASPP的乳腺癌细胞株，分析过表达iASPP对顺铂（cis-dichlorodiamine platinum

DDP）作用下乳腺癌细胞的影响。方法采用RT-PCR方法在乳腺组织中扩增出iASPP，转染入MCF-7，G418筛选获得过表达iASPP的乳腺癌细胞株，MTT和流式细胞仪分析过表达iASPP的乳腺癌细胞株在DDP作用下增殖和凋亡情况。结果成功构建了过表达iASPP的乳腺癌细胞株

过表达iASPP可明显降低DDP对乳腺癌细胞增殖的抑制作用（P0.01），并降低细胞凋亡的百分数(P0.01)。结论过表达iASPP可以明显降低乳腺癌细胞MCF-7对DDP作用的敏感性。

Abstract

Objective To analyze the effects of overexpressed iASPP on breast cancer cell line MCF-7. Methods The iASPP cDNA was obtained from breast carcinoma tissue by RT- PCR. The breast cancer cell line MCF-7 that overexpressed iASPP was established by stable transfection and G418 selection. The proliferation and apoptosis of the MCF-7 cells that overexpressed iASPP after exposure to cisplatin were detected by MTT and flow cytometry. Results The MCF-7 cells that overexpressed iASPP were successfully constructed. Overexpressed iASPP could significantly decrease the apoptosis rate of MCF-7 cells exposed to cisplatin and weaken the inhibitory effects of DDP on the proliferation of MCF-7 cells. Conclusion Overexpressed iASPP attenuates the sensitivity of human breast cancer cell line MCF-7 to cisplatin.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Amifostine enhances the resistance of renal proximal tubular epithelial cells to cisplatin by upregulating PKM2

Oxymatrine combined with cisplatin enhances expression of vascular endothelial growth factor and CD31 in subcutaneous xenografts of human hepatocellular carcinoma in nude mice

Role of O-6-methylguanine-DNA methyltransferase in cisplatinactivated autophagy in human gastric cancer SGC-7901 cells

Trichostatin A enhances cisplatin-induced cytotoxicity through inhibiting base excision repair of AP endonuclease in lung adenocarcinoma cells

Lactobacilli culture supernatant enhances sensitivity of triplenegative breast cancer cells to cisplatin via down-regulation of Piwil2

Related Author

薛嵘

马金刚

李莹屏

张健

高佩娟

黄文辉

杨晓军

李小丽

Related Institution

No data

⁰