Expression changes of NF-κB in aorta of rats with chronic renal failure

冯兵; 杨旭; 叶自林; 袁发焕; 杨惠标; 张耀全

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Expression changes of NF-κB in aorta of rats with chronic renal failure

更新时间：2026-01-09

- Expression changes of NF-κB in aorta of rats with chronic renal failure
- Journal of Army Medical University Vol. 30, Issue 15, Pages: 1430-1433(2008)
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  CLC： R322.121;{R392.13};R692.5
- Published：2008
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冯兵,杨旭,叶自林,袁发焕,杨惠标,张耀全.慢性肾衰竭大鼠主动脉NF-κB的表达变化[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1430-1433.

　FENG Bing,YANG Xu,YE Zi-lin,YUAN Fa-huan,YANG Hui-biao,ZHANG Yao-quan.Expression changes of NF-κB in aorta of rats with chronic renal failure[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1430-1433.
冯兵,杨旭,叶自林,袁发焕,杨惠标,张耀全.慢性肾衰竭大鼠主动脉NF-κB的表达变化[J].陆军军医大学学报(原第三军医大学学报),2008,30(15):1430-1433. DOI：

　FENG Bing,YANG Xu,YE Zi-lin,YUAN Fa-huan,YANG Hui-biao,ZHANG Yao-quan.Expression changes of NF-κB in aorta of rats with chronic renal failure[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(15):1430-1433. DOI：

摘要

目的探讨尿毒症时主动脉局部炎症信号NF-κB表达变化。方法采用单肾切除、对侧肾动脉部分结扎法建立慢性肾衰竭模型，RT-PCR检测NF-κB p65亚基、I-κB mRNA表达水平，免疫组织化学检测p65亚基蛋白表达，凝胶迁移率法测定NF-κB活性，并用图像分析定量。结果与正常大鼠比较，慢性肾衰竭大鼠主动脉NF-κB p65亚基mRNA表达水平显著升高(P0.01)，而I-κB mRNA表达水平显著降低(P0.01)，NF-κB p65亚基蛋白表达和活性也显著增加(P0.01)，并随病程延长而进一步上升。蛋白酶体抑制剂MG-132可明显抑制慢性肾衰竭大鼠主动脉NF-κB组分表达和活性升高。结论慢性肾衰竭大鼠主动脉NF-κB炎症信号通路存在显著活化。

Abstract

Objective To investigate the alteration of inflammatory signal(NF-κB) in aorta of rats with chronic renal failure. Methods The animal model was established by partial ligation of renal pedical artery and other side nephrectomy. The mRNA expression of NF-κB and I-κB were detected by RT-PCR

the protein expression of NF-κB was measured by immunohistochemistry

and the activity of NF-κB was examed by electrophoresis mobility shift assay(EMSA). Results Significant increase of the mRNA expression of NF-κB p65 was found in experimental group than in control group（P0.01）

while marked decrease of the mRNA expression of I-κB was noted（P0.01). The protein expression and activity of NF-κB was also increased significantly(P0.01)

and it is further increased in the course of renal failure progress. The expression and activity of NF-κB was inhibited significantly by MG-132 treatment. Conclusion The inflammatory signal of NF-κB is activated in the pathogenesis of chronic renal failure of rats.

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Urinary Nephropathy Center， the Second Affiliated Hospital of Chongqing Medical University

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