Objective    To investigate the regeneration of axons after the incomplete optic nerve injury in vivo treated by intravitreous injection of mixed crystallin.     Methods    Long Evans rats aged 3 months were subjected to the optic nerve clamping of both eyes

and the right eyes underwent intravitreous injection of mixed crystallin while the left eyes injected with isotonic saline served as controls. After injury

wheat germ agglutinin (WGA) anterograde tracing and electron microscopy were applied to evaluate axonal regeneration of retinal gangloin cells. F-VEP was performed before the injury and every time point after injury.     Results    Treatment with intravitreous injection of mixed crystallin resulted in more axonal fiber tracts regenerating and traversing the injured optic nerves. The amplitude of F-VEP in crystallin-treated group showed obvious recovery on 4-8 weeks after the injury

compared with the control group (P0.01).     Conclusion    Intravitreous injection of mixed crystallin can clear the path for axons to sprout

improve the regeneration of the axons and partly restore the visual function.