Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics

张涛; 汤为学; 蔡辉; 李少林

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Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics

更新时间：2026-01-09

- Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics
- Journal of Army Medical University Vol. 30, Issue 18, Pages: 1726-1729(2008)
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  CLC： R73-354;R730.23;R737.9
- Published：2008
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张涛,汤为学,蔡辉,李少林.不同转移潜能乳腺癌细胞亚系的建立与生物学特性的分析[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1726-1729.

　ZHANG Tao,TANG Wei-xue,CAI Hui,LI Shao-lin.Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(18):1726-1729.
张涛,汤为学,蔡辉,李少林.不同转移潜能乳腺癌细胞亚系的建立与生物学特性的分析[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1726-1729. DOI：

　ZHANG Tao,TANG Wei-xue,CAI Hui,LI Shao-lin.Establishment of breast cancer cell sublines with different potential of metastasis and their biological characteristics[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(18):1726-1729. DOI：

摘要

目的探讨利用细胞穿透人工基质膜能力的差异筛选不同转移潜能乳腺癌细胞的可行性，并分析二者生物学特性差异。方法人乳腺癌细胞系MDA-MB-435s经过连续人工基质膜侵袭试验后获得高、低转移潜能细胞株，并应用细胞生长曲线测定、侵袭力、黏附率实验，促血管形成实验分析所筛选出的不同转移潜能乳腺癌细胞的生物学性质的差异。结果获得2株转移潜能不同的乳腺癌细胞株，它们具有差异明显的体外生长速度、侵袭力、黏附力和促血管形成能力。结论利用细胞穿透人工基底膜能力的差异能够筛选出高、低转移潜能的乳腺癌细胞。

Abstract

Objective To investigate the validity of selecting subclones of human breast cancer cells with high- and low-metastatic potentiality

based on their different ability of penetrating the matrigel in vitro

and analyze their biological characteristics. Methods Continuous in vitro invasion assays in matrigel were applied to screen two subclones of human breast cancer cell line MDA-MB-435s

characterized by high- and low-metastatic potentiality. Cell growth curve assay

invasion assay

adhesion assay and angiopoiesis-promoting assay were conducted to explore the different biological properties of these two subclone cells. Results Two subclones of MDA-MB-435s

of high- and low-metastatic potentiality respectively

were obtained through continuous in vitro invasion assay

manifesting significant difference in growth rate

invasion ability

adhesion ability and angiopoiesis-promoting ability. Conclusion It is feasible to obtain subclones of human breast cancer cells of high- and low-metastatic potentiality by utilizing their difference in penetrating the matrigel in vitro.

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Related Institution

Department of Breast and Thyroid Surgery， First Affiliated Hospital， Army Medical University （Third Military Medical University）

State Key Laboratory of Trauma, Burns and Combined Injury, Department of Anti-Radiation Medicine, Chongqing Engineering Research Center for Nanomedicine, Institute of Combined Injury, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Department of Breast and Thyroid Surgery, the Second Affiliated Hospital of Chongqing Medical University

Department of Environmental Health, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Institute of Toxicology, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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