Noncanonical Wnt5a/Ca<sup>2+</sup> pathway activated by exogenous Wnt5a in K562 cells

李招权; 司维柯; 邹全明; 袁媛; 潘静; 赵宸

您当前的位置：

首页 >

文章列表页 >

Noncanonical Wnt5a/Ca²⁺ pathway activated by exogenous Wnt5a in K562 cells

更新时间：2026-01-09

- Noncanonical Wnt5a/Ca²⁺ pathway activated by exogenous Wnt5a in K562 cells
- Journal of Army Medical University Vol. 30, Issue 10, Pages: 924-927(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R730.33;R733.7
- Published：2008
- 稿件说明：
移动端阅览
李招权,司维柯,邹全明,袁媛,潘静,赵宸.外源性Wnt5a激活K562细胞非经典Wnt5a/Ca2+信号途径研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):924-927.

　LI Zhao-quan,SI Wei-ke,ZOU Quan-ming,YUAN Yuan,PAN Jing,ZHAO Chen.Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):924-927.
李招权,司维柯,邹全明,袁媛,潘静,赵宸.外源性Wnt5a激活K562细胞非经典Wnt5a/Ca2+信号途径研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(10):924-927. DOI：

　LI Zhao-quan,SI Wei-ke,ZOU Quan-ming,YUAN Yuan,PAN Jing,ZHAO Chen.Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(10):924-927. DOI：

摘要

目的观察外源性Wat5a对K562细胞非经典Wnt5a/Ca

途径的影响，进一步研究外源性Wnt5a诱导K562细胞的分化机制。方法激光共聚焦显微镜观察Ca

内流的变化，以RT-PCR及免疫组化检测β-catenin的表达变化，以Western blot检测cyclin D1的表达变化。结果外源性Wnt5a能明显促进K562细胞Ca

内流，显著下调cyclin D1表达，且能下调K562细胞β-catenin表达，但β-catenin表达下调无统计学意义。结论外源性Wnt5a能激活K562细胞非经典Wnt5a/Ca

途径，外源性Wnt5a诱导K562细胞分化机制可能与此相关。

Abstract

Objective To study the mechanism of K562 cell differentiation induced by exogenous Wnt5a in aspect of noncanonical Wnt5a/Ca

signaling pathway. Methods After K562 cells were cultured in medium supplemented with Wnt5a- or GFP-riched supernatant for 1-5 d

inflow changes were observed by laser confocal microscopy. The expression changes of β-catenin and cyclin D1 were detected by RT-PCR

immunocytochemical staining and Western blotting. Results Exogenous Wnt5a obviously promoted the Ca

inflow of K562 cells and down-regulated the expression of cyclin D1. Furthermore

the expression of β-catenin was lower

but had no statistically significance. Conclu

sion Exogenous Wnt5a can activate noncanonical Wnt5a/Ca

signaling pathway in K562 cells

which may relate to the K562 cell differentiation.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simvastatin down-regulates mRNA expressions of MAPK1, cyclin D1, E2F1 and c-myc in K562 cells

Effect of TNTs-mediated mitochondrial transfer between mesenchymal stem cells and endothelial cells on vascularization in vitro

Ethacrynic acid promotes apoptosis in lung cancer A549 cells when combined with cisplatin chemotherapy

Effect of DIM on inhibiting proliferation and invasion in human gastric carcinoma SGC-7901 cells

Effect of targeted Sox2 silencing on expression of β-catenin and TIF1γ in human breast cancer MDA-MB-231 cells

Related Author

杨永长

传良敏

黄文芳

刘华

曾娅莉

胥国强

刘文

何云影

Related Institution

No data

⁰

Noncanonical Wnt5a/Ca2+ pathway activated by exogenous Wnt5a in K562 cells

DOI：

摘要

Abstract

关键词

Keywords

references

Noncanonical Wnt5a/Ca²⁺ pathway activated by exogenous Wnt5a in K562 cells