Objective    Endothelial lipase (EL) modulates the monocyte adhesion to the endothelial cells. We explore the effect of EL on the expression of adhesion molecules in endothelial cells.     Methods    Human umbilical vein endothelial cells (HUVECs) were treated with TNF-α (10 ng/ml)

TNF-α (10 ng/ml)+anti-EL antibody (0

2.5

5

10

25

50 ng/ml

respectively) or serum-free media

then the expression levels of intercellular adhesion molecule-1 (ICAM-1) mRNA were detected by RT-PCR.     Results    After treated with TNF-α

the ICAM-1 mRNA levels expressed by HUVECs were significantly increased. These effects were significantly antagonized by anti-EL antibody in a dose-dependent manner.      Conclusion    EL expressed in HUVECs stimulated by TNF-α can promote the expressions of adhesion molecules in endothelial cells

indicating EL may participate in  the pathophysiologic progress of coronary artery disease.