Objective    To investigate the effects of mouse amniotic fluid on the proliferation and apoptosis of mouse hepatoma cell line H

22

.     Methods    Treated with the mixture of RPMI 1640 medium and 10% mouse amniotic fluid collected respectively on gestational day 11

13

15 and 17 or RPMI 1640 medium as control

the proliferation of H

22

cells was detected with MTT and the apoptosis of H

22

cells with Annexin V-FITC; H

22

cells were observed morphologically under ele

ctron microscope; The expressions of proliferating cell nuclear antigen (PCNA) and p53 in H

22

cells were investigated with immunofluorescence staining (S-P method) and laser confocal microscopy.     Results    Amniotic fluid significantly inhibited the cell proliferation as well as the PCNA expressions

increased the p53 expressions and induced cell apoptosis. The amniotic fluid collected on gestational day 11 was the most effective

while that of gestational day 17 was the least effective (P0.05)

and their effects were both in a time-dependent manner. There was a negative correlation between PCNA and p53 (r=-0.808

P0.01).     Conclusion    Amniotic fluid collected on gestational day 11

13

15 and 17 can inhibit the proliferation of H

22

cells and induce their apoptosis. The earlier pregnancy and the longer intervention is

more significant effects of amniotic fluid are.