Transfection of maspin/PCR2.1 vector inhibits uPA and uPAR expressions in gastric cancer cells in vitro

周文文; 易永芬; 郑爱华

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Transfection of maspin/PCR2.1 vector inhibits uPA and uPAR expressions in gastric cancer cells in vitro

更新时间：2026-01-09

- Transfection of maspin/PCR2.1 vector inhibits uPA and uPAR expressions in gastric cancer cells in vitro
- Journal of Army Medical University Vol. 30, Issue 12, Pages: 1132-1135(2008)
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  CLC： R394-33;R730.23;R735.2
- Published：2008
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周文文,易永芬,郑爱华.转染maspin cDNA在胃癌细胞中的表达及其对uPA和uPAR表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1132-1135.

　ZHOU Wen-wen,YI Yong-fen,ZHENG Ai-hua.Transfection of maspin/PCR2.1 vector inhibits uPA and uPAR expressions in gastric cancer cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1132-1135.
周文文,易永芬,郑爱华.转染maspin cDNA在胃癌细胞中的表达及其对uPA和uPAR表达的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1132-1135. DOI：

　ZHOU Wen-wen,YI Yong-fen,ZHENG Ai-hua.Transfection of maspin/PCR2.1 vector inhibits uPA and uPAR expressions in gastric cancer cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1132-1135. DOI：

摘要

目的研究转染maspin cDNA在胃癌细胞中的表达及其对uPA和uPAR表达的影响，探讨maspin基因抑制胃癌浸润转移的机制。方法构建 maspin/PCR2．1表达载体

转染胃癌细胞株MKN28和SGC7901

用RT-PCR和Western blot检测maspin基因

uPA和uPAR表达的变化。结果经酶切证实，得到正向插入的真核表达质粒maspin/PCR2．1。成功转染到MKN28和SGC7901细胞中并表达，uPA和uPAR的mRNA和蛋白表达均降低。结论成功构建了maspin/PCR2．1表达载体，maspin基因可在靶细胞表达，并能抑制uPA和uPAR的表达。

Abstract

Objective To investigate the inhibitory effect of maspin gene on metastasis and invasion of gastric cancer and its mechanism. Methods Maspin gene was ligated to the expression vector PCR2.1 with T

DNA ligase after the PCR2.1 was digested by HindⅢ/XbaⅠ. The recombinant vector maspin/PCR2.1 was transfected into human gastric cancer cell lines MKN28 and SGC7901

then the mRNA and protein expression changes of maspin gene

uPA and uPAR were detected respectively by PT-PCR and Western blotting. Results After identified by digestion and sequencing

the reconstructed plasmid was confirmed to contain the correct and full nucleotide sequence of maspin gene. The expressions of maspin gene were up-regulated in MK

N28 and SGC7901 cell lines

while the expressions of uPA and uPAR were down-regulated. Conclusion The expression vector maspin/PCR2.1 is constructed successfully and can be expressed in eukaryotic cells. Expressions of uPA and uPAR can be inhibited by maspin gene.

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