Effect of insulin-like grow factor 1 on early regeneration of rat sciatic nerve

李光振; 朱刚; 陈菁; 冯华; 王宪荣

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Effect of insulin-like grow factor 1 on early regeneration of rat sciatic nerve

更新时间：2026-01-09

- Effect of insulin-like grow factor 1 on early regeneration of rat sciatic nerve
- Journal of Army Medical University Vol. 30, Issue 11, Pages: 1025-1027(2008)
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  CLC： R322.85;R329.28;R651.302
- Published：2008
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李光振,朱刚,陈菁,冯华,王宪荣.IGF-1对大鼠坐骨神经再生早期的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(11):1025-1027.

　LI Guang-zhen,ZHU Gang,CHEN Jing,FENG Hua,WANG Xian-rong.Effect of insulin-like grow factor 1 on early regeneration of rat sciatic nerve[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):1025-1027.
李光振,朱刚,陈菁,冯华,王宪荣.IGF-1对大鼠坐骨神经再生早期的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(11):1025-1027. DOI：

　LI Guang-zhen,ZHU Gang,CHEN Jing,FENG Hua,WANG Xian-rong.Effect of insulin-like grow factor 1 on early regeneration of rat sciatic nerve[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(11):1025-1027. DOI：

摘要

目的探讨胰岛素样生长因子-1对大鼠坐骨神经再生早期的影响。方法建立大鼠坐骨神经切割伤模型，通过硅胶管进行桥接，实验组于再生室内注入胰岛素样生长因子-1，对照组注入等量生理盐水，于术后3、5、7、9、11 d取材，用SABC免疫组化法检测轴突再生情况以及雪旺细胞数目、形态和排列方式的变化。结果 ①雪旺细胞：在对照组，术后3 d，断端间隙内仅可见少量雪旺细胞，术后7 d，雪旺细胞数量明显增多，术后11 d，整个间隙内的雪旺细胞排列呈有序的条索状。在实验组，术后3 d，雪旺细胞即明显增多，以后各相同时相点雪旺细胞数目均明显高于对照组。②再生轴突：在对照组，术后5 d，轴突开始长出，术后7 d轴突快速增长，至第11天，再生的轴突已长过5 mm的间隙到达神经远端。在实验组，术后3 d轴突即开始长出，9 d就快到达远端。结论胰岛素样生长因子-1能促进轴突延伸和雪旺细胞增殖，明显促进坐骨神经再生。

Abstract

Objective To investigate the role of insulin-like grow factor 1 (IGF-1) on the regeneration of peripheral nerve after operation. Methods The right sciatic nerve of adult female SD rats was transected and bridged with silicone tube. IGF-1 was injected into the silicone tube in the treated group

while normal saline instead in control group

with 30 rats in each group. On postoperative day 3

9 and 11

the regeneration of axons was observed by SABC immunohistochemical methods with anti-neurofilment 200

and the proliferation of Schwann cells was measured with anti-S-100. Results Significant difference was seen in the length of regenerating neurites and the number of Schwann cells at all time points between two groups (P0.01). Conclusion Local application of IGF-1 in the regenerative chamber after transaction promotes the proliferation of Schwann cells and the growth of neurites.

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