Significance of scavenger receptor A in rat liver tissues during acute obstructive suppurative cholangitis

杨慷; 刘作金; 赵蕾; 龚建平

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Significance of scavenger receptor A in rat liver tissues during acute obstructive suppurative cholangitis

更新时间：2026-01-09

- Significance of scavenger receptor A in rat liver tissues during acute obstructive suppurative cholangitis
- Journal of Army Medical University Vol. 30, Issue 18, Pages: 1714-1717(2008)
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  CLC： R322.47;R392.3;R575.702
- Published：2008
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杨慷,刘作金,赵蕾,龚建平.急性梗阻性化脓性胆管炎时大鼠肝组织清道夫受体-A的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1714-1717.

　YANG Kang,LIU Zuo-jin,ZHAO Lei,GONG Jian-ping.Significance of scavenger receptor A in rat liver tissues during acute obstructive suppurative cholangitis[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(18):1714-1717.
杨慷,刘作金,赵蕾,龚建平.急性梗阻性化脓性胆管炎时大鼠肝组织清道夫受体-A的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1714-1717. DOI：

　YANG Kang,LIU Zuo-jin,ZHAO Lei,GONG Jian-ping.Significance of scavenger receptor A in rat liver tissues during acute obstructive suppurative cholangitis[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(18):1714-1717. DOI：

摘要

目的观测急性梗阻性化脓性胆管炎（acute obstructive suppurative cholangitis

AOSC）时大鼠肝组织中清道夫受体-A（scavenger receptor A

SR-A）的表达及其与炎症介质的产生和肝组织损害的关系。方法结扎大鼠胆总管，于胆总管内注入大肠杆菌O111∶B4（菌落浓度5×109 cfu/ml），建立急性胆管炎动物模型。测定血浆中内毒素，观测不同时相点肝组织中SR-A mRNA 及蛋白质的表达及肝组织病理形态学变化，同时测定TNF-α含量变化，并与结扎胆总管注射生理盐水组、假手术组比较。结果在急性胆管炎组中，随着梗阻感染时间的延长和血浆内毒素浓度的升高，血浆中TNF-α含量明显增加，肝组织中SR-A 的mRNA和蛋白质的表达明显下降，与对照组比较有统计学差异（P0.01）。同时可见随着处理时间延长而逐渐加重的肝组织损伤的病理改变。结论 AOSC时，随着肝组织中SR-A表达的下调，枯否细胞（Kupffer cells

KCs）清除、灭活内毒素的能力下降，同时KCs激活释放的细胞因子增多，诱发并加重肝组织的损害。

Abstract

Objective To investigate the expression of scavenger receptor A (SR-A) in rat liver tissues during acute obstructive suppurative cholangitis (AOSC) and its relations with inflammatory mediators and hepatic injury. Methods Wistar rat models of AOSC were reproduced by ligating choledochus and inside injecting Escherichia coli O111∶B4. The plasma endotoxin concentrations

TNF-α levels and expressions of SR-A protein and mRNA in liver tissues were assayed by limulus test

ELISA

immunohistochemistry and RT-PCR

respectively. Pathological changes of liver tissues were observed under light microscope. Results With the time prolonged

the plasma endotoxin concentrations and the TNF-α levels gradually increased

but the SR-A protein and mRNA expressions decreased in the rats of experimental group

significantly different with those in sham operation group and normal saline group (P0.05). Meanwhile

the hepatic injury was gradually aggravated. Conclusion With the decreased expression of SR-A in liver tissues during AOSC

the Kupffer cells’ abilities of clearing and inactivating endotoxin are down-regulated

and simultaneously Kupffer cells produce and release more cytokines that may initiate and aggravate liver injury.

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