MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro

佘强; 邓松柏; Uta C.Hoppe

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MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro

更新时间：2026-01-09

- MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro
- Journal of Army Medical University Vol. 30, Issue 12, Pages: 1129-1131(2008)
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  CLC： R322.11;R329.25;R331.38
- Published：2008
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佘强,邓松柏,Uta CHoppe.MiRP1对异源转染的CHO细胞上HCN1和HCN2通道电生理特性的调节[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1129-1131.

　SHE Qiang,DENG Song-bai,Uta C. Hoppe.MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1129-1131.
佘强,邓松柏,Uta CHoppe.MiRP1对异源转染的CHO细胞上HCN1和HCN2通道电生理特性的调节[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1129-1131. DOI：

　SHE Qiang,DENG Song-bai,Uta C. Hoppe.MiRP1 regulates electrophysiological properties of HCN1 and HCN2 in CHO cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1129-1131. DOI：

摘要

目的评价Mink相关肽1（MiRP1）对异源转染的CHO细胞上的HCN1、HCN2通道电生理特性的影响。方法将MiRP1质粒DNA分别和HCN1、HCN2质粒DNA共转染CHO-K1细胞，用全细胞膜片钳记录细胞膜上的HCN通道电流。结果 MiRP1显著增加HCN2 [(37.8±4.8) pA/pF（n=11）vs (25.9±1.7) pA/pF（n=10）; -140 mV

P0.05]的电流密度，但是对HCN1的电流大小没有影响[(41.3±10.9) pA/pF（n=13） vs (34.9±7.8) pA/pF（n=11）; P0.05]；MiRP1可加快HCN1[时间常数：(180.9±8.6) ms vs (306.1±45.6) ms; -80 mV

P0.05]和HCN2 [时间常数：(391.8±33.6) ms vs (763.5±106.1) ms; -80 mV

P0.05]激活动力学；MiRP1对HCN1及HCN2通道激活的电压依赖性没有影响。结论 MiRP1可加快HCN1和HCN2通道激活，且增加HCN2的电流表达。

Abstract

Objective To investigate the modulation of electrophysiological properties of hyperpolarization-activated cyclic nucleotide-gated channels(HCN) HCN1 and HCN2 by transfecting MinK-related peptide 1(MiRP1) in Chinese hamster ovarian (CHO) cells. Methods CHO cells were co-transfected with plasmid DNA encoding either of the cardiac HCN isoforms (HCN1

HCN2) with MiRP1 to observe the effect of MiRP1 on HCN whole-cell currents. Whole-cell patch clamp technique was used to record the 2 channel currents of the transfected cells. Results MiRP1 significantly increased whole-cell current density of HCN2 [(37.8±4.8) pA/pF（n=11） vs control (25.9±1.7) pA/pF（n=10）; at -140 mV

P0.05]

while not affecting HCN1 current density [(41.3±10.9) pA/pF（n=13） vs control (34.9±7.8) pA/pF（n=11）; P0.05]. Moreover

MiRP1 accelerated the activation kinetic of HCN1 [tau (180.9±8.6) ms vs control (306.1±45.6) ms; at -80 mV

P0.05] and HCN2 [tau (391.8±33.6) ms vs control (763.5±106.1) ms; at -80 mV

P0.05].But MiRP1 did not affect voltage dependence of HCN1 and HCN2 channel activation. Conclusion MiRP accelerates HCN1 or HCN2 channel activation kinetics

and leads to increase of HCN2 current density.

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