Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells

钟大平; 李焱; 周进明; 周琪; 姜友昭; 杨启红; 梁后杰

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Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells

更新时间：2026-01-09

- Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells
- Journal of Army Medical University Vol. 30, Issue 17, Pages: 1607-1610(2008)
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  CLC： R394.3;R73-362;R735.35
- Published：2008
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钟大平,李焱,周进明,周琪,姜友昭,杨启红,梁后杰.Tiam1基因沉默降低结肠癌细胞VEGF-C/-D的表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1607-1610.

　ZHONG Da-ping,LI Yan,ZHOU Jin-ming,ZHOU Qi,JIANG You-zhao,YANG Qi-hong,LIANG Hou-jie.Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1607-1610.
钟大平,李焱,周进明,周琪,姜友昭,杨启红,梁后杰.Tiam1基因沉默降低结肠癌细胞VEGF-C/-D的表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1607-1610. DOI：

　ZHONG Da-ping,LI Yan,ZHOU Jin-ming,ZHOU Qi,JIANG You-zhao,YANG Qi-hong,LIANG Hou-jie.Silenced Tiam1 gene inhibits the expressions of VEGF-C/-D in colorectal cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1607-1610. DOI：

摘要

目的探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)的表达与结肠癌淋巴管生成的关系。方法将实验细胞分为3组

分别为未干扰组（HCT116）、空质粒载体对照组(HCT116/CN)和干扰组(HCT116/siRNA-Tiam1)。应用siRNA使Tiam1基因表达下调

通过RT-PCR检测Tiam1 mRNA表达抑制率

并用RT-PCR和Western blot方法检测Tiam1基因干扰前后VEGF-C/-D mRNA和蛋白的表达情况。结果通过筛选后稳定表达的情况下

siRNA组的Tiam1、VEGF-C/-D mRNA和蛋白的表达明显低于未干扰组。结论 Tiam1参与了VEGF-C/-D表达的调节，推测Tiam1可能通过诱导VEGF-C/-D的表达影响结肠癌淋巴管的生成。Tiam1可作为结肠癌淋巴转移过程中一个有价值的指标。

Abstract

Objective To study the relationship between Tiam1 gene and lymphangiogenesis in colorectal cancer. Methods Tiam1 was silenced by siRNA

then linked with pGenesil-EGFP1

finally transfected into the colorectal cancer cells HCT116. The cells without transfection and transfected with blank vector served as controls. The Tiam1 mRNA expressions were detected by RT-PCR. The expressions of VEGF-C/-D mRNA and protein were detected by RT-PCR and Western blot. Results The expressions of Tiam1 and VEGF-C/-D in HCT116 cells with Tiam1 gene silenced were lower than those in control groups. Conclusion Tiam1 can affect lymphangiogensis in colon cancer by its effect on VEGF-C/-D. It may be a useful marker for lymphatic metastasis in colorectal cancer.

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