Screening differently expressed genes of mouse stem cells C3H10T1/2 after Wnt3A adenovirus infection: a result with cDNA microarray assay

朱丹; 谢鹏; 康权; 黄文; 曾志磊; 牟君; 魏有东; 陈晓; 彭丹; 刘庆军

您当前的位置：

首页 >

文章列表页 >

Screening differently expressed genes of mouse stem cells C3H10T1/2 after Wnt3A adenovirus infection: a result with cDNA microarray assay

更新时间：2026-01-09

- Screening differently expressed genes of mouse stem cells C3H10T1/2 after Wnt3A adenovirus infection: a result with cDNA microarray assay
- Journal of Army Medical University Vol. 30, Issue 17, Pages: 1604-1606(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.2;R394-33;R394.3
- Published：2008
- 稿件说明：
移动端阅览
朱丹,谢鹏,康权,黄文,曾志磊,牟君,魏有东,陈晓,彭丹,刘庆军.基因芯片分析Wnt刺激后小鼠多潜能分化干细胞C3H10T1/2的差异基因表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1604-1606.

　ZHU Dan,XIE Peng,KANG Quan,HUANG Wen,ZENG Zhi-lei,MU Jun,WEI You-dong,CHEN Xiao,PENG Dan,LIU Qing-jun.Screening differently expressed genes of mouse stem cells C3H10T1/2 after Wnt3A adenovirus infection: a result with cDNA microarray assay[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1604-1606.
朱丹,谢鹏,康权,黄文,曾志磊,牟君,魏有东,陈晓,彭丹,刘庆军.基因芯片分析Wnt刺激后小鼠多潜能分化干细胞C3H10T1/2的差异基因表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1604-1606. DOI：

　ZHU Dan,XIE Peng,KANG Quan,HUANG Wen,ZENG Zhi-lei,MU Jun,WEI You-dong,CHEN Xiao,PENG Dan,LIU Qing-jun.Screening differently expressed genes of mouse stem cells C3H10T1/2 after Wnt3A adenovirus infection: a result with cDNA microarray assay[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1604-1606. DOI：

摘要

目的探讨Wnt信号传导系统对诱导干细胞向神经系统分化的基因表达的影响。方法采用最新的含有22 000多个已知基因及EST克隆的小鼠基因芯片U74Av2，检测了Wnt刺激后的小鼠多潜能分化干细胞C3H10T1/2中目标基因表达的变化，并进行实时定量PCR，验证了基因芯片结果。结果 Wnt刺激后小鼠多潜能分化干细胞C3H10T1/2中Wnt信号传导系统各主要组成基因表达有不同程度增加；对干细胞向神经系统发育有潜在调节作用的基因表达也有增加或减少。结论表达谱芯片分析成功筛选出Wnt刺激引起表达改变且可能与神经发生有关的基因。

Abstract

Objective To explore the effect of Wnt signaling pathway on neural differentiation of stem cells. Methods Multiple target genes were analyzed in mouse stem cells C3H10T1/2 by mouse gene chip U74Av2

a novel microarray possessed over 22 000 target genes. Real time PCR was applied for the validation of microarray result. Results The expression of major components of Wnt signaling pathway was increased to various extent. The expression of some potential genes related to neurogenesis increased

the others decreased. Conclusion With cDNA microarray technology

specific genes related to neurogenesis were screened successfully

the expression of which were regulated by Wnt in mouse stem cells C3H10T1/2.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Microarray analysis of the mechanism of liver injury induced by acute cadmium exposure in rats

DNA methylation microarray-based screening of epigenetically masked genes in patients with anti-tuberculosis drug-induced liver injury

S100p promotes tamoxifen resistance and migration in human breast cancer MCF-7 cells

DNA chip construction for β-thalassemia and achondroplasia

Gene expression profile of lung tissues in rats with high altitude pulmonary edema

Related Author

Huifeng PI

Zhengping YU

Li TIAN

Jia XIE

Mengyan CHEN

Ping DENG

Tiesheng HAN

Yuhong LI

Related Institution

Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Hebei Provincial Key Laboratory of Coal Industry Occupational Health and Safety, School of Public Health, North China University of Science and Technology

Department of Clinical Laboratory, Hulun Buir Infectious Disease Hospital, Hulun, Buir, Inner Mongolia Autonomous Region

⁰