Objective    To study the effects of angiotensin Ⅱ (AngⅡ)

as inducing agent in vitro

in inducing adult adipose-derived mesenchymal stem cells (ADMSCs) to the direction of myocardial cell.     Methods    ADMSCs were isolated and cultured from adult adipose tissues. Different concentrations of AngⅡ (0.05

0.10 and 0.20  μmol/L

respectively) were used to induce ADMSCs. Untreated ADMSCs were used as normal control. The cell morphological feature was observed under inverted phase-contrast microscope and transmission electron microscope. Immunocytochemical staining was used to detect the cardiac troponin-I (cTn-I) and calculate the conversion rate.     Results    After three weeks of induction

cTn-I-positive cells could be detected in 0.20  μmol/L AngⅡ group. Four weeks later

cTn-I-positive cells were detected in 0.10 and 0.20  μmol/L AngⅡ groups

furthermore

the number of cTn-I-positive cells in 0.20  μmol/L AngⅡ group was significantly higher than that of other groups.     Conclusion    Ang Ⅱ can induce in vitro ADMSCs to differentiate into cardiocyte-like cells

and the best induction concentration is 0.20  μmol/L.