Objective    To construct the transformed mouse BaF3-P210 cell line stably expressing BCR/ABL and to initially investigate the influence of BCR/ABL on the cell biological characteristics of BaF3 cell line.     Methods    The retroviral vector with bcr/abl gene was transfected into the packaging cell line. The BaF3 cells were infected with the collected viral supernatant. The transgenic BaF3-P210 cell line stably expressing BCR/ABL were screened and subcloned. The integration of the bcr/abl gene

in the genome of the target cell was determined by PCR and DNA sequencing

trypan blue staining assay

flow cytometry and MTT assay.     Results    The bcr/abl gene was integrated into the BaF3 cell genome; RT-PCR and Western blot verified the stable expression of the bcr/abl gene and protein in the screened subclone cell line BaF3-P210. Compared with the control group

the cell proliferation rate was promoted (P0.05)

and the cell proportion in G

0

/G

1

phase was decreased with the increasing of the cell proportion in both S and G

2

/M phase (P0.05); the cell proliferation inhibition rate and the early apoptosis rate of the BaF3-P210 cells were respectively (54.08±1.90)％(P0.05) and (12.35±2.04)％ (P0.05) after the treatment of protein tyrosine kinase inhibitor STI571.     Conclusion    The transformed mouse BaF3-P210 cell line stably expressing BCR/ABL was successfully constructed. The expression of BCR/ABL not only promoted the proliferation

but also accelerated the cell cycle progression of the BaF3 cells. It endowed the BaF3-P210 transformed cell line with the sensitivity to STI571.