Construction and immunogenicity of a fusion vaccine candidate of UreB-OMP18-LTB from H. pylori

葛迪; 郭刚; 毛旭虎; 张卫军; 邹全明

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Construction and immunogenicity of a fusion vaccine candidate of UreB-OMP18-LTB from H. pylori

更新时间：2026-01-09

- Construction and immunogenicity of a fusion vaccine candidate of UreB-OMP18-LTB from H. pylori
- Journal of Army Medical University Vol. 30, Issue 12, Pages: 1107-1110(2008)
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  CLC： R378.99;R392.1;R394-33
- Published：2008
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葛迪,郭刚,毛旭虎,张卫军,邹全明.幽门螺杆菌UreB414-OMP18-LTB融合蛋白的构建及免疫原性初步研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1107-1110.

　GE Di,GUO Gang,MAO Xu-hu,ZHANG Wei-jun,ZOU Quan-ming.Construction and immunogenicity of a fusion vaccine candidate of UreB-OMP18-LTB from H. pylori[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1107-1110.
葛迪,郭刚,毛旭虎,张卫军,邹全明.幽门螺杆菌UreB414-OMP18-LTB融合蛋白的构建及免疫原性初步研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(12):1107-1110. DOI：

　GE Di,GUO Gang,MAO Xu-hu,ZHANG Wei-jun,ZOU Quan-ming.Construction and immunogenicity of a fusion vaccine candidate of UreB-OMP18-LTB from H. pylori[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(12):1107-1110. DOI：

摘要

目的构建幽门螺杆菌(Helicobacter pylori

Hp) 尿素酶B亚单位活性片段（UreB414）、外膜蛋白（OMP18）及大肠埃希菌LTB融合疫苗并研究其抗原性。方法 PCR扩增ureb414、omp18、ltb基因，分别克隆入pMD18-T载体，测序并鉴定方向后依次连接，构建融合基因UOL，然后将其亚克隆入原核表达载体pET-28a，转化入大肠埃希菌BL21，SDS-PAGE电泳进行表达，Western印迹检测表达蛋白的抗原性。结果 PCR扩增分别获得约414、537 bp和320 bp产物，与GenBank中相关序列具有高度同源性，三者融合后获得一约1 200 bp目的基因。重组融合蛋白表达量可达细菌总蛋白的15％，免疫印迹检测结果显示该重组蛋白可为UreB、OMP18、LTB兔抗血清特异识别，具有良好的抗原性。结论成功构建了具有良好免疫原性的UreB-OMP18-LTB融合蛋白。

Abstract

Objective To construct a fusion vaccine of Helicobacter pylori (Hp) urease subunit B (UreB)

outer membrane protein18 (OMP18) and E.coli heat-labile enterotoxin B subunit

summarized as UOL and study its antigenicity so as to lay a foundation to produce an H.pylori vaccine. Methods The UreB414

OMP18 and LTB genes were amplified by PCR

and cloned into pMD 18-T vector

and the products were identified and fused to abtain UOL. UOL was then subcloned into a procaryon expression vector pET-28a and transformed into E. coli cells BL21. Immunogenicity of the recombinant fusion protein was analyzed by Western blotting. Results The PCR fragments of ureb414

omp18 and ltb genes were about 414

537 and 320 bp

which had high homology with those related sequences in GenBank. Recombinant protein amounted to 15% of the total bacterial protein. Western blot showed that the protein had a specific reaction with UreB

OMP18 and LTB antiserum. Conclusion Expression of the fusion protein of UOL in E. coli cells provides a basis to produce engineered vaccine against Hp.

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